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Scientific paper - Original scientific paper
Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron
Journal of Trace Elements in Medicine and Biology, 46 (2018); 62-75. https://doi.org/doi:10.1016/j.jtemb.2017.11.011
Sabolić, Ivan; Škarica, Mario; Ljubojević, Marija; Breljak, Davorka; Herak-Kramberger, Carol M.; Crljen, Vladiana; Ljubešić, Nikola

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Sabolić, I., Škarica, M., Ljubojević, M., Breljak, D., Herak Kramberger, C. M., Crljen, V. & Ljubešić, N. (2018). Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron. Journal of Trace Elements in Medicine and Biology, 46., 62-75. doi: doi:10.1016/j.jtemb.2017.11.011

Sabolić, Ivan, et al. "Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron." Journal of Trace Elements in Medicine and Biology, vol. 46, 2018, pp. 62-75. https://doi.org/doi:10.1016/j.jtemb.2017.11.011

Sabolić, Ivan, Mario Škarica, Marija Ljubojević, Davorka Breljak, Carol M. Herak Kramberger, Vladiana Crljen and Nikola Ljubešić. "Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron." Journal of Trace Elements in Medicine and Biology 46 (2018): 62-75. https://doi.org/doi:10.1016/j.jtemb.2017.11.011

Sabolić, I., et al. (2018) 'Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron', Journal of Trace Elements in Medicine and Biology, 46, pp. 62-75. doi: doi:10.1016/j.jtemb.2017.11.011

Sabolić I, Škarica M, Ljubojević M, Breljak D, Herak Kramberger CM, Crljen V, and sur.. Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron. Journal of Trace Elements in Medicine and Biology [Internet]. 2018 March [cited 2024 October 15];46:62-75. doi: doi:10.1016/j.jtemb.2017.11.011

I. Sabolić, et al., "Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron", Journal of Trace Elements in Medicine and Biology, vol. 46, pp. 62-75, March 2018. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:434036. [Accessed: 15 October 2024]

Metadata
Title (english)Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron
AuthorIvan Sabolić
AuthorMario Škarica
AuthorMarija Ljubojević
AuthorDavorka Breljak
AuthorCarol M. Herak-Kramberger
AuthorVladiana Crljen
AuthorNikola Ljubešić
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
Rodent kidneys exhibit three isoforms of metallothioneins (MTs), MT1, MT2 and MT3, with poorly characterized localization along the nephron. Here we studied in adult male Wistar rats the renal expression of MTs mRNA by end-point RT-PCR and MT proteins by immunochemical methods The expression pattern of MT1 mRNA was cortex (CO)>outer stripe (OS)=inner stripe (IS)=inner medulla (IM), of MT2 mRNA was IM>CO>IS=OS, and of MT3 mRNA was IM>CO=OS=IM. MT1/2-antibody stained with heterogeneous intensity the cell cytoplasm and nuclei in proximal tubule (PT) and thin ascending limb, whereas MT3-antibody stained weakly the cell cytoplasm in various cortical tubules and strongly the nuclei in all nephron segments. However, the isolated nuclei exhibited an absence of MT1/2 and presence of MT3 protein. In MT1/2-positive PT cells, the intracellular staining appeared diffuse or bipolar, but the isolated brush-border, basolateral and endosomal membranes were devoid of MT1/2 proteins. In the lumen of some PT profiles, the heterogeneously sized MT1/2-rich vesicles were observed, with the limiting membrane positive for NHE3, but negative for V-ATPase, CAIV, and megalin, whereas their interior was positive for CAII and negative for cytoskeleton. They seem to be pinched off from the luminal membrane of MT1/2-rich cells, as also indicated by transmission electron microscopy. We conclude that in male rats, MTs are heterogeneously abundant in the cell cytoplasm and/or nuclei along the nephron. The MT1/2-rich vesicles in the tubule lumen may represent a source of urine MT and membranous material, whereas MT3 in nuclei may handle zink and locally-produced reactive oxygen species.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionAccepted version
Journal titleJournal of Trace Elements in Medicine and Biology
Numberingvol. 46, pp. 62-75
p-ISSN0946-672X
DOIhttps://doi.org/doi:10.1016/j.jtemb.2017.11.011
URN:NBNurn:nbn:hr:105:434036
Publication2018-03
Document URLhttp://doi.org/10.1016/j.jtemb.2017.11.011
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 10:13:49