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Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron
Journal of Trace Elements in Medicine and Biology, 46 (2018); 62-75. https://doi.org/doi:10.1016/j.jtemb.2017.11.011
Sabolić, Ivan; Škarica, Mario; Ljubojević, Marija; Breljak, Davorka; Herak-Kramberger, Carol M.; Crljen, Vladiana; Ljubešić, Nikola

Institucijski repozitorij: Dr Med - digitalni repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

Citirajte ovaj rad

Sabolić, I., Škarica, M., Ljubojević, M., Breljak, D., Herak Kramberger, C. M., Crljen, V. i Ljubešić, N. (2018). Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron. Journal of Trace Elements in Medicine and Biology, 46., 62-75. doi: doi:10.1016/j.jtemb.2017.11.011

Sabolić, Ivan, et al. "Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron." Journal of Trace Elements in Medicine and Biology, vol. 46, 2018, str. 62-75. https://doi.org/doi:10.1016/j.jtemb.2017.11.011

Sabolić, Ivan, Mario Škarica, Marija Ljubojević, Davorka Breljak, Carol M. Herak Kramberger, Vladiana Crljen i Nikola Ljubešić. "Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron." Journal of Trace Elements in Medicine and Biology 46 (2018): 62-75. https://doi.org/doi:10.1016/j.jtemb.2017.11.011

Sabolić, I., et al. (2018) 'Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron', Journal of Trace Elements in Medicine and Biology, 46, str. 62-75. doi: doi:10.1016/j.jtemb.2017.11.011

Sabolić I, Škarica M, Ljubojević M, Breljak D, Herak Kramberger CM, Crljen V, i sur.. Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron. Journal of Trace Elements in Medicine and Biology [Internet]. 03.2018. [pristupljeno 29.05.2024.];46:62-75. doi: doi:10.1016/j.jtemb.2017.11.011

I. Sabolić, et al., "Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron", Journal of Trace Elements in Medicine and Biology, vol. 46, str. 62-75, Ožujak 2018. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:434036. [Citirano: 29.05.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:105:434036
Podaci o radu
Naslov (engleski)Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron
AutorIvan Sabolić
AutorMario Škarica
AutorMarija Ljubojević
AutorDavorka Breljak
AutorCarol M. Herak-Kramberger
AutorVladiana Crljen
AutorNikola Ljubešić
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Sažetak (engleski)
Rodent kidneys exhibit three isoforms of metallothioneins (MTs), MT1, MT2 and MT3, with poorly characterized localization along the nephron. Here we studied in adult male Wistar rats the renal expression of MTs mRNA by end-point RT-PCR and MT proteins by immunochemical methods The expression pattern of MT1 mRNA was cortex (CO)>outer stripe (OS)=inner stripe (IS)=inner medulla (IM), of MT2 mRNA was IM>CO>IS=OS, and of MT3 mRNA was IM>CO=OS=IM. MT1/2-antibody stained with heterogeneous intensity the cell cytoplasm and nuclei in proximal tubule (PT) and thin ascending limb, whereas MT3-antibody stained weakly the cell cytoplasm in various cortical tubules and strongly the nuclei in all nephron segments. However, the isolated nuclei exhibited an absence of MT1/2 and presence of MT3 protein. In MT1/2-positive PT cells, the intracellular staining appeared diffuse or bipolar, but the isolated brush-border, basolateral and endosomal membranes were devoid of MT1/2 proteins. In the lumen of some PT profiles, the heterogeneously sized MT1/2-rich vesicles were observed, with the limiting membrane positive for NHE3, but negative for V-ATPase, CAIV, and megalin, whereas their interior was positive for CAII and negative for cytoskeleton. They seem to be pinched off from the luminal membrane of MT1/2-rich cells, as also indicated by transmission electron microscopy. We conclude that in male rats, MTs are heterogeneously abundant in the cell cytoplasm and/or nuclei along the nephron. The MT1/2-rich vesicles in the tubule lumen may represent a source of urine MT and membranous material, whereas MT3 in nuclei may handle zink and locally-produced reactive oxygen species.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeZavršna verzija rukopisa prihvaćena za objavljivanje (postprint)
Naslov časopisaJournal of Trace Elements in Medicine and Biology
Brojčani podacivol. 46, str. 62-75
p-ISSN0946-672X
DOIhttps://doi.org/doi:10.1016/j.jtemb.2017.11.011
URN:NBNurn:nbn:hr:105:434036
Datum objave publikacije2018-03
URL dokumentahttp://doi.org/10.1016/j.jtemb.2017.11.011
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2024-03-28 10:13:49