prikaz prve stranice dokumenta Targeting tumor markers with antisense peptides: an example of human prostate specific antigen
  Download
PDF 9.47 MB
Scientific paper - Original scientific paper
Targeting tumor markers with antisense peptides: an example of human prostate specific antigen
International Journal of Molecular Sciences, 20 (2019), 9; https://doi.org/10.3390/ijms20092090
Štambuk, Nikola; Konjevoda, Paško; Turčić, Petra; Šošić, Hrvoje; Aralica, Gorana; Babić, Damir; Seiwerth, Sven; Kaštelan, Željko; Kujundžić, Renata Novak; Wardega, Piotr; Žutelija, Jelena Barać; Gračanin, Ana Gudelj; Gabričević, Mario

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

Cite this document

Štambuk, N., Konjevoda, P., Turčić, P., Šošić, H., Aralica, G., Babić, D. ... Gabričević, M. (2019). Targeting tumor markers with antisense peptides: an example of human prostate specific antigen. International Journal of Molecular Sciences, 20. (9). doi: 10.3390/ijms20092090

Štambuk, Nikola, et al. "Targeting tumor markers with antisense peptides: an example of human prostate specific antigen." International Journal of Molecular Sciences, vol. 20, no. 9, 2019. https://doi.org/10.3390/ijms20092090

Štambuk, Nikola, Paško Konjevoda, Petra Turčić, Hrvoje Šošić, Gorana Aralica, Damir Babić, Sven Seiwerth, et al. "Targeting tumor markers with antisense peptides: an example of human prostate specific antigen." International Journal of Molecular Sciences 20, no. 9 (2019). https://doi.org/10.3390/ijms20092090

Štambuk, N., et al. (2019) 'Targeting tumor markers with antisense peptides: an example of human prostate specific antigen', International Journal of Molecular Sciences, 20(9). doi: 10.3390/ijms20092090

Štambuk N, Konjevoda P, Turčić P, Šošić H, Aralica G, Babić D, and sur.. Targeting tumor markers with antisense peptides: an example of human prostate specific antigen. International Journal of Molecular Sciences [Internet]. 2019 April 28 [cited 2025 January 13];20(9). doi: 10.3390/ijms20092090

N. Štambuk, et al., "Targeting tumor markers with antisense peptides: an example of human prostate specific antigen", International Journal of Molecular Sciences, vol. 20, no. 9, April 2019. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:903196. [Accessed: 13 January 2025]

Metadata
Title (english)Targeting tumor markers with antisense peptides: an example of human prostate specific antigen
AuthorNikola Štambuk
AuthorPaško Konjevoda
AuthorPetra Turčić
AuthorHrvoje Šošić
AuthorGorana Aralica
AuthorDamir Babić
AuthorSven Seiwerth
AuthorŽeljko Kaštelan
AuthorRenata Novak Kujundžić
AuthorPiotr Wardega
AuthorJelena Barać Žutelija
AuthorAna Gudelj Gračanin
AuthorMario Gabričević
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
The purpose of this paper was to outline the development of short peptide targeting of the human prostate specific antigen (hPSA), and to evaluate its effectiveness in staining PSA in human prostate cancer tissue. The targeting of the hPSA antigen by means of antisense peptide AVRDKVG was designed according to a three-step method involving: 1. The selection of the molecular target (hPSA epitope), 2. the modeling of an antisense peptide (paratope) based on the epitope sequence, and 3. the spectroscopic evaluation of sense-antisense peptide binding. We then modified standard hPSA immunohistochemical staining practice by using a biotinylated antisense peptide instead of the standard monoclonal antibody and compared the results of both procedures. Immunochemical testing on human tissue showed the applicability of the antisense peptide technology to human molecular targets. This methodology represents a new approach to deriving peptide ligands and potential lead compounds for the development of novel diagnostic substances, biopharmaceuticals and vaccines.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleInternational Journal of Molecular Sciences
Numberingvol. 20, no. 9
p-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms20092090
URN:NBNurn:nbn:hr:105:903196
Publication2019-04-28
Document URLhttp://doi.org/10.3390/ijms20092090
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 10:35:44