prikaz prve stranice dokumenta Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption
  Download
PDF 1.45 MB
Scientific paper - Original scientific paper
Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption
Journal of Biological Chemistry, 281 (2006), 35; 25509-25521. https://doi.org/10.1074/jbc.M513276200
Šimić, Petra; Buljan Culej, Jasminka; Orlić, Iva; Grgurević, Lovorka; Drača, Nataša; Spaventi, Radan; Vukičević, Slobodan

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

Cite this document

Šimić, P., Buljan Culej, J., Orlić, I., Grgurević, L., Drača, N., Spaventi, R. & Vukičević, S. (2006). Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption. Journal of Biological Chemistry, 281. (35), 25509-25521. doi: 10.1074/jbc.M513276200

Šimić, Petra, et al. "Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption." Journal of Biological Chemistry, vol. 281, no. 35, 2006, pp. 25509-25521. https://doi.org/10.1074/jbc.M513276200

Šimić, Petra, Jasminka Buljan Culej, Iva Orlić, Lovorka Grgurević, Nataša Drača, Radan Spaventi and Slobodan Vukičević. "Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption." Journal of Biological Chemistry 281, no. 35 (2006): 25509-25521. https://doi.org/10.1074/jbc.M513276200

Šimić, P., et al. (2006) 'Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption', Journal of Biological Chemistry, 281(35), pp. 25509-25521. doi: 10.1074/jbc.M513276200

Šimić P, Buljan Culej J, Orlić I, Grgurević L, Drača N, Spaventi R, and sur.. Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption. Journal of Biological Chemistry [Internet]. 2006 September 01 [cited 2024 May 06];281(35):25509-25521. doi: 10.1074/jbc.M513276200

P. Šimić, et al., "Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption", Journal of Biological Chemistry, vol. 281, no. 35, pp. 25509-25521, September 2006. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:368042. [Accessed: 06 May 2024]

Metadata
Title (english)Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption
AuthorPetra Šimić
AuthorJasminka Buljan Culej
AuthorIva Orlić
AuthorLovorka Grgurević
AuthorNataša Drača
AuthorRadan Spaventi
AuthorSlobodan Vukičević
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Although recombinant human bone morphogenetic proteins (BMPs) are used locally for treating bone defects in humans, their systemic effect on bone augmentation has not been explored. We have previously demonstrated that demineralized bone (DB) from ovariectomized (OVX) rats cannot induce bone formation when implanted ectopically at the subcutaneous site. Here we showed in vitro that 17beta-estradiol (E2) specifically induced expression of Bmp6 mRNA in MC3T3-E1 preosteoblastic cells and that bone extracts from OVX rats lack BMPs. Next we demonstrated that 125I-BMP-6 administered systemically accumulated in the skeleton and also restored the osteoinductive capacity of ectopically implanted DB from OVX rats. BMP-6 applied systemically to aged OVX rats significantly increased bone volume and mechanical characteristics of both the trabecular and cortical bone, the osteoblast surface, serum osteocalcin and osteoprotegerin levels, and decreased the osteoclast surface, serum C-telopeptide, and interleukin-6. E2 was significantly less effective, and was not synergistic with BMP-6. Animals that discontinued BMP-6 therapy maintained bone mineral density gains for another 12 weeks. BMP-6 increased in vivo the bone expression of Acvr-1, Bmpr1b, Smad5, alkaline phosphatase, and collagen type I and decreased expression of Bmp3 and BMP antagonists, chordin and cerberus. These results show, for the first time, that systemically administered BMP-6 restores the bone inductive capacity, microarchitecture, and quality of the skeleton in osteoporotic rats.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionAccepted version
Journal titleJournal of Biological Chemistry
Numberingvol. 281, no. 35, pp. 25509-25521
p-ISSN0021-9258
DOIhttps://doi.org/10.1074/jbc.M513276200
URN:NBNurn:nbn:hr:105:368042
Publication2006-09-01
Document URLhttp://www.jbc.org/content/281/35/25509.long
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 11:34:21