prikaz prve stranice dokumenta Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption
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Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption
Journal of Biological Chemistry, 281 (2006), 35; 25509-25521. https://doi.org/10.1074/jbc.M513276200
Šimić, Petra; Buljan Culej, Jasminka; Orlić, Iva; Grgurević, Lovorka; Drača, Nataša; Spaventi, Radan; Vukičević, Slobodan

Institucijski repozitorij: Dr Med - digitalni repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

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Šimić, P., Buljan Culej, J., Orlić, I., Grgurević, L., Drača, N., Spaventi, R. i Vukičević, S. (2006). Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption. Journal of Biological Chemistry, 281. (35), 25509-25521. doi: 10.1074/jbc.M513276200

Šimić, Petra, et al. "Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption." Journal of Biological Chemistry, vol. 281, br. 35, 2006, str. 25509-25521. https://doi.org/10.1074/jbc.M513276200

Šimić, Petra, Jasminka Buljan Culej, Iva Orlić, Lovorka Grgurević, Nataša Drača, Radan Spaventi i Slobodan Vukičević. "Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption." Journal of Biological Chemistry 281, br. 35 (2006): 25509-25521. https://doi.org/10.1074/jbc.M513276200

Šimić, P., et al. (2006) 'Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption', Journal of Biological Chemistry, 281(35), str. 25509-25521. doi: 10.1074/jbc.M513276200

Šimić P, Buljan Culej J, Orlić I, Grgurević L, Drača N, Spaventi R, i sur.. Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption. Journal of Biological Chemistry [Internet]. 01.09.2006. [pristupljeno 15.05.2024.];281(35):25509-25521. doi: 10.1074/jbc.M513276200

P. Šimić, et al., "Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption", Journal of Biological Chemistry, vol. 281, br. 35, str. 25509-25521, Rujan 2006. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:368042. [Citirano: 15.05.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:105:368042
Podaci o radu
Naslov (engleski)Systemically administered bone morphogenetic protein-6 restores bone in aged ovariectomized rats by increasing bone formation and suppressing bone resorption
AutorPetra Šimić
AutorJasminka Buljan Culej
AutorIva Orlić
AutorLovorka Grgurević
AutorNataša Drača
AutorRadan Spaventi
AutorSlobodan Vukičević
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Although recombinant human bone morphogenetic proteins (BMPs) are used locally for treating bone defects in humans, their systemic effect on bone augmentation has not been explored. We have previously demonstrated that demineralized bone (DB) from ovariectomized (OVX) rats cannot induce bone formation when implanted ectopically at the subcutaneous site. Here we showed in vitro that 17beta-estradiol (E2) specifically induced expression of Bmp6 mRNA in MC3T3-E1 preosteoblastic cells and that bone extracts from OVX rats lack BMPs. Next we demonstrated that 125I-BMP-6 administered systemically accumulated in the skeleton and also restored the osteoinductive capacity of ectopically implanted DB from OVX rats. BMP-6 applied systemically to aged OVX rats significantly increased bone volume and mechanical characteristics of both the trabecular and cortical bone, the osteoblast surface, serum osteocalcin and osteoprotegerin levels, and decreased the osteoclast surface, serum C-telopeptide, and interleukin-6. E2 was significantly less effective, and was not synergistic with BMP-6. Animals that discontinued BMP-6 therapy maintained bone mineral density gains for another 12 weeks. BMP-6 increased in vivo the bone expression of Acvr-1, Bmpr1b, Smad5, alkaline phosphatase, and collagen type I and decreased expression of Bmp3 and BMP antagonists, chordin and cerberus. These results show, for the first time, that systemically administered BMP-6 restores the bone inductive capacity, microarchitecture, and quality of the skeleton in osteoporotic rats.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeZavršna verzija rukopisa prihvaćena za objavljivanje (postprint)
Naslov časopisaJournal of Biological Chemistry
Brojčani podacivol. 281, br. 35, str. 25509-25521
p-ISSN0021-9258
DOIhttps://doi.org/10.1074/jbc.M513276200
URN:NBNurn:nbn:hr:105:368042
Datum objave publikacije2006-09-01
URL dokumentahttp://www.jbc.org/content/281/35/25509.long
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2024-03-28 11:34:21