prikaz prve stranice dokumenta Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment
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Scientific paper - Original scientific paper
Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment
International Journal of Molecular Sciences, 24 (2023), 18; 14161. https://doi.org/10.3390/ijms241814161
Petrović, Dražen Juraj; Jagečić, Denis; Krasić, Jure; Sinčić, Nino; Mitrečić, Dinko

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Petrović, D. J., Jagečić, D., Krasić, J., Sinčić, N. & Mitrečić, D. (2023). Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment. International Journal of Molecular Sciences, 24. (18). doi: 10.3390/ijms241814161

Petrović, Dražen Juraj, et al. "Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment." International Journal of Molecular Sciences, vol. 24, no. 18, 2023. https://doi.org/10.3390/ijms241814161

Petrović, Dražen Juraj, Denis Jagečić, Jure Krasić, Nino Sinčić and Dinko Mitrečić. "Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment." International Journal of Molecular Sciences 24, no. 18 (2023). https://doi.org/10.3390/ijms241814161

Petrović, D. J., et al. (2023) 'Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment', International Journal of Molecular Sciences, 24(18). doi: 10.3390/ijms241814161

Petrović DJ, Jagečić D, Krasić J, Sinčić N, Mitrečić D. Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment. International Journal of Molecular Sciences [Internet]. 2023 September 15 [cited 2024 December 27];24(18). doi: 10.3390/ijms241814161

D. J. Petrović, D. Jagečić, J. Krasić, N. Sinčić and D. Mitrečić, "Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment", International Journal of Molecular Sciences, vol. 24, no. 18, September 2023. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:820198. [Accessed: 27 December 2024]

Metadata
Title (english)Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment
AuthorDražen Juraj Petrović
AuthorDenis Jagečić
AuthorJure Krasić
AuthorNino Sinčić
AuthorDinko Mitrečić
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Histology and Embryology)
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Medical Biology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Abstract (english)
In vitro cell culture is a routinely used method which is also applied for in vitro modeling of various neurological diseases. On the other hand, media used for cell culture are often not strictly standardized between laboratories, which hinders the comparison of the obtained results. Here, we compared the effects of homocysteine (Hcy), a molecule involved in neurodegeneration, on immature cells of the nervous system cultivated in basal medium or media supplemented by either fetal bovine serum or basic fibroblast growth factor. The number of cells in basal media supplemented with basic fibroblast growth factor (bFGF) was 2.5 times higher in comparison to the number of cells in basal media supplemented with fetal bovine serum (FBS). We also found that the neuron-specific β-3-tubulin protein expression dose dependently decreased with increasing Hcy exposure. Interestingly, bFGF exerts a protective effect on β-3-tubulin protein expression at a concentration of 1000 µM Hcy compared to FBS-treated neural stem cells on Day 7. Supplementation with bFGF increased SOX2 protein expression two-fold compared to FBS supplementation. GFAP protein expression increased five-fold on Day 3 in FBS-treated neural stem cells, whereas on Day 7, bFGF increased GFAP expression two-fold compared to FBS-treated neural stem cells. Here, we have clearly shown that the selection of culturing media significantly influences various cellular parameters, which, in turn, can lead to different conclusions in experiments based on in vitro models of pathological conditions.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleInternational Journal of Molecular Sciences
Numberingvol. 24, no. 18, 14161
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms241814161
URN:NBNurn:nbn:hr:105:820198
Publication2023-09-15
ProjectNumber: KK.01.1.1.01.0007
Title (croatian): Znanstveni centar izvrsnosti - eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi
Title (english): Scientific center of excellence - Experimental and clinical research of hypoxic-ischemic brain damage in perinatal and adulthood
Acronym: ZCI-NEURO
Leader: Miloš Judaš
Jurisdiction: eu
Funding stream: European Regional Development Fund
Document URLhttps://www.mdpi.com/1422-0067/24/18/14161
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2024-05-21 08:48:38