prikaz prve stranice dokumenta Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment
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Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment
International Journal of Molecular Sciences, 24 (2023), 18; 14161. https://doi.org/10.3390/ijms241814161
Petrović, Dražen Juraj; Jagečić, Denis; Krasić, Jure; Sinčić, Nino; Mitrečić, Dinko

Institucijski repozitorij: Dr Med - digitalni repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

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Petrović, D. J., Jagečić, D., Krasić, J., Sinčić, N. i Mitrečić, D. (2023). Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment. International Journal of Molecular Sciences, 24. (18). doi: 10.3390/ijms241814161

Petrović, Dražen Juraj, et al. "Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment." International Journal of Molecular Sciences, vol. 24, br. 18, 2023. https://doi.org/10.3390/ijms241814161

Petrović, Dražen Juraj, Denis Jagečić, Jure Krasić, Nino Sinčić i Dinko Mitrečić. "Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment." International Journal of Molecular Sciences 24, br. 18 (2023). https://doi.org/10.3390/ijms241814161

Petrović, D. J., et al. (2023) 'Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment', International Journal of Molecular Sciences, 24(18). doi: 10.3390/ijms241814161

Petrović DJ, Jagečić D, Krasić J, Sinčić N, Mitrečić D. Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment. International Journal of Molecular Sciences [Internet]. 15.09.2023. [pristupljeno 22.11.2024.];24(18). doi: 10.3390/ijms241814161

D. J. Petrović, D. Jagečić, J. Krasić, N. Sinčić i D. Mitrečić, "Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment", International Journal of Molecular Sciences, vol. 24, br. 18, Rujan 2023. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:820198. [Citirano: 22.11.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:105:820198
Podaci o radu
Naslov (engleski)Effect of Fetal Bovine Serum or Basic Fibroblast Growth Factor on Cell Survival and the Proliferation of Neural Stem Cells: The Influence of Homocysteine Treatment
AutorDražen Juraj Petrović
AutorDenis Jagečić
AutorJure Krasić
AutorNino Sinčić
AutorDinko Mitrečić
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA HISTOLOGIJU I EMBRIOLOGIJU)
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA MEDICINSKU BIOLOGIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Citologija, histologija i embriologija
Sažetak (engleski)
In vitro cell culture is a routinely used method which is also applied for in vitro modeling of various neurological diseases. On the other hand, media used for cell culture are often not strictly standardized between laboratories, which hinders the comparison of the obtained results. Here, we compared the effects of homocysteine (Hcy), a molecule involved in neurodegeneration, on immature cells of the nervous system cultivated in basal medium or media supplemented by either fetal bovine serum or basic fibroblast growth factor. The number of cells in basal media supplemented with basic fibroblast growth factor (bFGF) was 2.5 times higher in comparison to the number of cells in basal media supplemented with fetal bovine serum (FBS). We also found that the neuron-specific β-3-tubulin protein expression dose dependently decreased with increasing Hcy exposure. Interestingly, bFGF exerts a protective effect on β-3-tubulin protein expression at a concentration of 1000 µM Hcy compared to FBS-treated neural stem cells on Day 7. Supplementation with bFGF increased SOX2 protein expression two-fold compared to FBS supplementation. GFAP protein expression increased five-fold on Day 3 in FBS-treated neural stem cells, whereas on Day 7, bFGF increased GFAP expression two-fold compared to FBS-treated neural stem cells. Here, we have clearly shown that the selection of culturing media significantly influences various cellular parameters, which, in turn, can lead to different conclusions in experiments based on in vitro models of pathological conditions.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaInternational Journal of Molecular Sciences
Brojčani podacivol. 24, br. 18, 14161
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms241814161
URN:NBNurn:nbn:hr:105:820198
Datum objave publikacije2023-09-15
ProjektŠifra: KK.01.1.1.01.0007
Naziv (hrvatski): Znanstveni centar izvrsnosti - eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi
Naziv (engleski): Scientific center of excellence - Experimental and clinical research of hypoxic-ischemic brain damage in perinatal and adulthood
Kratica: ZCI-NEURO
Voditelj: Miloš Judaš
Pravna nadležnost: eu
Financijer: Europska komisija
Linija financiranja: European Regional Development Fund
URL dokumentahttps://www.mdpi.com/1422-0067/24/18/14161
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2024-05-21 08:48:38