prikaz prve stranice dokumenta Morfologija živaca kože i ponašanje povezano s boli u štakorskom modelu šećerne bolesti tipa 1 i 2
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master's thesis
Morfologija živaca kože i ponašanje povezano s boli u štakorskom modelu šećerne bolesti tipa 1 i 2
Split: University of Split, School of Medicine, 2013. urn:nbn:hr:171:275184
Škopljanac, Ivan
University of Split
School of Medicine

Institutional repository: MEFST Repository

Cite this document

Škopljanac, I. (2013). Morfologija živaca kože i ponašanje povezano s boli u štakorskom modelu šećerne bolesti tipa 1 i 2 (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:275184

Škopljanac, Ivan. "Morfologija živaca kože i ponašanje povezano s boli u štakorskom modelu šećerne bolesti tipa 1 i 2." Master's thesis, University of Split, School of Medicine, 2013. https://urn.nsk.hr/urn:nbn:hr:171:275184

Škopljanac, Ivan. "Morfologija živaca kože i ponašanje povezano s boli u štakorskom modelu šećerne bolesti tipa 1 i 2." Master's thesis, University of Split, School of Medicine, 2013. https://urn.nsk.hr/urn:nbn:hr:171:275184

Škopljanac, I. (2013). 'Morfologija živaca kože i ponašanje povezano s boli u štakorskom modelu šećerne bolesti tipa 1 i 2', Master's thesis, University of Split, School of Medicine, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:171:275184

Škopljanac I. Morfologija živaca kože i ponašanje povezano s boli u štakorskom modelu šećerne bolesti tipa 1 i 2 [Master's thesis]. Split: University of Split, School of Medicine; 2013 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:171:275184

I. Škopljanac, "Morfologija živaca kože i ponašanje povezano s boli u štakorskom modelu šećerne bolesti tipa 1 i 2", Master's thesis, University of Split, School of Medicine, Split, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:171:275184

Metadata
TitleMorfologija živaca kože i ponašanje povezano s boli u štakorskom modelu šećerne bolesti tipa 1 i 2
Title (english)Morphology of cutaneous nerves and pain-related behaviour in rat models of diabetes type 1 and 2
AuthorIvan Škopljanac
MentorLivia Puljak (mentor)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2013, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Abstract
Cilj istraživanja: Istražiti promjene kože štakora u STZ modelu dijabetesa tipa 2 i usporediti ih s promjenama u modelu dijabetesa tipa 1 te ispitati ponašanje štakora povezano s boli. Materijal i metode: Model DM1 induciran je intraperitonealnom injekcijom streptozotocina (STZ) u dozi od 55 mg/kg, a model DM2 je induciran kombinacijom manje doze STZ-a (35 mg/kg) i masne hrane (udio masti 58%). Za analizu ponašanja povezanog s boli korišteni su toplinski i mehanički podražaji. Štakori su žrtvovani dva mjeseca nakon induciranja dijabetesa, a zatim je analizirana debljina epidermisa i gustoća intraepidermalnih živčanih vlakana Rezultati: Dva mjeseca nakon indukcije dijabetesa uočeno je statistički značajno stanjenje epidermisa stražnje šape u dijabetičkom modelu tipa 1 i tipa 2 u odnosu na njihove kontrolne skupine. Samo DM-1 skupina je razvila povećanu osjetljivost na hladnoću, toplinu i mehanički podražaj. Prosječan broj intraepidermalnih živčanih vlakana je statistički značajno smanjen u DM1 i DM2 skupini u usporedbi sa pripadajućim kontrolnim skupinama Zaključci: Šećerna bolest tip 1 i tip 2 uzrokuju značajno smanjenje debljine epidermisa i broja intraepidermalnih živčanih vlakana u štakorskim modelima. Te su promjene značajno više prisutne kod tipa 1 šećerne bolesti. U dijabetesu tip 1 nalazimo ponašanje povezano uz bol koje nije prisutno u tip 2 dijabetesu. Štakorski modeli dijabetesa tipa 1 mogao bi se koristiti za farmakološka istraživanja u kojima se provjerava djelotvornost tvari za prevenciju nastanka neuropatskih promjena.
Abstract (english)
Objectives: To study cutaneous changes in rat models of diabetes type 1 and 2 and compare them to pain-related behaviour. Material and methods: Diabetes type 1 (DM1) model was induced with 55 mg/kg of streptozotocin (STZ) and for induction of diabetes type 2 (DM2) a combination of high fat diet and low-dose STZ (35 mg/kg) was used. Pain-related behavior was analyzed using thermal and mechanical stimuli. Two weeks and two months following induction of diabetes rats were sacrificed. Epidermal thickness with intraepidermal nerve fiber quantity were analyzed in skin samples. Results: Two months after diabetes induction significant epidermal thinning was observed in hind paw in diabetic rats of type 1 and type 2 compared to their controls respectively. Only DM-1 group exhibited increased pain-related behavior with sensitivity to thermal and mechanical stimuli. Intraepidermal nerve fiber density was significantly lower in DM1 and DM2 groups compared to their respective controls. Conclusions: Diabetes type 1 and 2 cause significant epidermal thinning and depletion of intraepidermal nerve fibers in rat models. These changes are significantly more prominent in diabetes type 1. Increased pain-related behavior was present in diabetes type 1, but not in diabetes type 2 models. Rat model of diabetes type 1 could be used for pharmacological research, for evaluating efficacy of therapeutics for diabetic neuropathy.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:275184
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2016-05-02 07:42:39