Objective: The aim of this study was to describe the life habits of pregnant women in Dalmatia and to compare the habits between primiparous and multiparous women. Materials and methods: A cross-sectional study was conducted among pregnant women who came for a screening combined test at the Clinic for Women's Diseases and Obstetrics of the Clinical Hospital Center Split. A total of 173 pregnant women participated, of whom 183 were offered a questionnaire. Successive sample collection was conducted from October 2019 to March 2020. The questionnaire examined the life habits of pregnant women, which include cigarette smoking and alcohol consumption, eating habits, physical activity, sleep habits and psychological characteristics of the respondents. Kolmogorov – Smirnov test, chi-square test, Mann-Whitney U test, student t-test, Spearman correlation rank test were used in the statistical analysis. In the case of a P value less than 0.05, the result was considered statistically significant. The SPSS statistics program (IBM SPSS Statistics v21) was used for data analysis. Results: Pregnant women from this study were classified by parity into the group of primiparous and multiparous women. A statistically significant difference was found in the representation of the Mediterranean diet between the mentioned groups. The median MDSS sum for multi-born women was 10.0 (5.0), while for first-born women it was 7.0 (6.0) (P = 0.013). Comparing physical activity with respect to parity, no statistically significant difference was found between the groups. In terms of sleep habits, during the working part of the week, primiparous women slept 30 minutes longer per day than multiparous women (P = 0.017). The median self-assessment of quality of life was better in primiparous women and was 9.0 (1.0) in contrast to multiparous women with 8.0 (2.0) (P = 0.021). Body mass index was significantly negatively correlated with health self-assessment (r = -0.216, P = 0.007), sleep duration during working days (r = -0.168, P = 0.034) and sleep duration during days off (r = -0.175, P = 0.027). Stress perception was statistically significantly negatively correlated with quality of life (r = -0.330, P <0.001), feelings of happiness (r = -0.409, P <0.001) and optimism (r = -0.374, P <0.001), and positively with anxiety (r = 0.482, P <0.001). Conclusion: Multiparous women adhered more to the pattern of the Mediterranean diet than primiparous women. Physical activity and eating habits are not satisfactory in any of the examined groups. Primiparous women sleep longer than multiparous women. According to the unexpectedly bad lifestyle habits of the examined groups, it is necessary to work on additional education and promotion of a healthy lifestyle.