Destination management companies (DMC) play a very important role in the tourist development of a certain destination. They create complex tourist products of a higher value, also by raising the quality of tourist offer of such destination. There are numerous tourist segments of consumers on the tourist market. For each market segment on the tourist market destination management companies should develop appropriate offer, taking into consideration specific needs and wishes. Tourism should become accessible to all, and tourist destination should ensure for each tourist, no matter the age, gender, possibilities or needs, to enjoy equally in all tourist services. The accessible tourism can be defined as the way of tourism that takes care of all consumers who can enjoy in tourist experiences no matter the type of their disability. In other to make it possible for the destination management companies to make their services accessible, it is neccessary to understand the possibilities and needs of the market segment of the disabled. Within that context, the aim of this thesis is to analyse the challenges of business activities of destination management companies in accessible tourism. In order to fulfill the objectives of the thesis the analysing of attitudes of the disabled in accordance with their habits and organisation of tourist trips and journeys as well as using of services of destination management companies when organising such journeys was performed. They especially appreciate the accessibility and regularity of information on accessible services and activities in destinations, taking into consideration that they are relatively satisfied with their collaboration with organizers of the journeys. In accordance with the facts stated among very important challenges of business activities of destination management activities within the area of accessible tourism the following needs to be mentioned: adjustment of products of destination management companies to accessible tourism, efficient communication with clients as well as education of employees of destination managmenet companies for the purpose of better understanding of clients' needs from the segment of accessible tourism. Besides the fact that destination management companies can through offering their products have influence on increase of accesible products offer and accessibility of information on them, they can also increase awareness on importance of accessibility that is actually the first step of insurance of equal possibilities for all participants of tourist movements.