Objectives: The aim of this paper is to determine the level of knowledge of preschool teachers in Split-Dalmatia County on first aid procedures and to assess whether there is a significant difference in the level of knowledge depending on years of work experience, place of work and employment establishment. Participants and methodology: The respondents were kindergarten teachers in Split-Dalmatia County with more than one year of work experience. All respondents were female and employed in state, private and religious kindergartens. The number of respondents involved in this study was 394. The paper uses graphical and tabular presentation methods, descriptive statistics, Wilcoxon's signed test for one independent sample, and Mann-Whitney U test. The conclusions were made at the level of significance of 5%. This analysis was done using MS EXCEL and STATISTICA 12 statistical software. Results: Having done the test, it was found that the high percentage of the teachers answered correctly to two groups of questions, i.e. a high level of knowledge was reported. The third group of questions resulted in a moderate level of knowledge, i.e. the mean value of correct answers is 50%. Kindergarten teachers in Split-Dalmatia County are aware of their inadequate knowledge of first aid procedures, as confirmed by the results. The average value of correct answers to the questions of The responsibility of the teachers in emergency situations is 50% with the central tendency ranging from 50% to 100% of correct answers, while the mean of the correct answers to First Aid Knowledge – I. and II. part is 40% with a central tendency ranging from 40% to 60% of correct answers. According to the place of work, the level of knowledge among the teachers in the city area is higher than in the countryside and on the islands (p = 0.005). As for the knowledge shown by the kindergarten teachers depending on the ownership of the educational establishment, a statistically significant difference was found in the knowledge measured by the groups Responsibility of the Educator and the Knowledge of First Aid - part I. A higher level of knowledge was shown by the teachers working in the kindergartens owned by a private person or a religious organization. Conclusion: Since injuries can affect child's mental and physical health, it is necessary that persons in charge of their safety take all necessary precautions to prevent them. This is expected from the kindergarten teachers, as well. Permanent education in first aid, follow-up with new health guidelines, constant children supervision and kindergarten safety check are the duties of every teacher.