prikaz prve stranice dokumenta Zaštitna uloga S-proteina pri liofilizaciji probiotičkihLactobacillus vrsta
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undergraduate thesis
Zaštitna uloga S-proteina pri liofilizaciji probiotičkihLactobacillus vrsta
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:603535
Sarajlić, Lana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Sarajlić, L. (2012). Zaštitna uloga S-proteina pri liofilizaciji probiotičkihLactobacillus vrsta (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:603535

Sarajlić, Lana. "Zaštitna uloga S-proteina pri liofilizaciji probiotičkihLactobacillus vrsta." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:603535

Sarajlić, Lana. "Zaštitna uloga S-proteina pri liofilizaciji probiotičkihLactobacillus vrsta." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:603535

Sarajlić, L. (2012). 'Zaštitna uloga S-proteina pri liofilizaciji probiotičkihLactobacillus vrsta', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:603535

Sarajlić L. Zaštitna uloga S-proteina pri liofilizaciji probiotičkihLactobacillus vrsta [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:159:603535

L. Sarajlić, "Zaštitna uloga S-proteina pri liofilizaciji probiotičkihLactobacillus vrsta", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:603535

Metadata
TitleZaštitna uloga S-proteina pri liofilizaciji probiotičkihLactobacillus vrsta
Title (english)Protective role of the S-protein duringfreeze-dryingof probioticLactobacillus strains
AuthorLana Sarajlić
MentorBlaženka Kos (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2012-06-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
L. buchneri 4t1, L. paraplantarum SF9B, L. brevis SF15B, L. brevis D6, L. brevis ZG1 i L. helveticus M92 su probiotički sojevi kod kojih je SDS-PAGE metodom dokazana prisutnost S-proteina. Pretpostavlja se da ovi proteini imaju važnu biološku ulogu jer bakterija troši znatni dio metaboličke aktivnosti stanice za njihovu sintezu. U ovom radu je provedena liofilizacija probiotičkih sojeva L. buchneri 4t1, L. paraplantarum SF9B, L. brevis SF15B, L. brevis D6, L. brevis ZG1 i L. helveticus M92, te je ispitan mogući zaštitni utjecaj S-proteina napreživljavanje ovih bakterija tijekom liofilizacije. Broj stanica prije i nakon liofilizacije odreĎen je indirektnom metodom. Rezultati su pokazali da prisutnost S-proteina smanjuje smrtnost stanica tijekom liofilizacije, što dokazuje zaštitnu ulogu ovog proteina te ga čini poželjnim kod probiotičkih sojeva.
Abstract (english)
Probiotic strains L. buchneri 4t1, L. paraplantarum SF9B, L. brevis SF15B, L. brevis D6, L. brevis ZG1 i L. helveticus M92 have a surface S-protein which was identified by SDS-PAGE. It is assumed that these proteins have an important biological role because bacteria consume a substantial part of the metabolic activity of cells for their synthesis. In this work the freeze-dryingof the wet biomass of the probiotic strains L. buchneri 4t1, L. paraplantarum SF9B, L. brevis SF15B, L. brevis D6, L. brevis ZG1 i L. helveticus M92 was performed and moreover an possible protective influence of the S-protein on the bacterial viability during freeze-drying was observed. The number of cells before and after freeze-drying was determined by indirect method and the percentage of the cell survival was calculated. The results have shown that the presence of S-protein reduces cell death during the freeze-drying,which provesthe protective role of this protein making it desirable for probiotic strains.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:603535
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
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Created on2017-05-16 09:20:32