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master's thesis
Hepatoprotektivni učinak polifenola iz ekstrakta trnine na antioksidacijski status u miša
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. urn:nbn:hr:159:490655
Paradžik, Ivona
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Food Quality Control
Laboratory for Food Chemistry and Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Paradžik, I. (2016). Hepatoprotektivni učinak polifenola iz ekstrakta trnine na antioksidacijski status u miša (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:490655

Paradžik, Ivona. "Hepatoprotektivni učinak polifenola iz ekstrakta trnine na antioksidacijski status u miša." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:490655

Paradžik, Ivona. "Hepatoprotektivni učinak polifenola iz ekstrakta trnine na antioksidacijski status u miša." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:490655

Paradžik, I. (2016). 'Hepatoprotektivni učinak polifenola iz ekstrakta trnine na antioksidacijski status u miša', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 19 May 2024, https://urn.nsk.hr/urn:nbn:hr:159:490655

Paradžik I. Hepatoprotektivni učinak polifenola iz ekstrakta trnine na antioksidacijski status u miša [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2016 [cited 2024 May 19] Available at: https://urn.nsk.hr/urn:nbn:hr:159:490655

I. Paradžik, "Hepatoprotektivni učinak polifenola iz ekstrakta trnine na antioksidacijski status u miša", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:159:490655

Metadata
TitleHepatoprotektivni učinak polifenola iz ekstrakta trnine na antioksidacijski status u miša
Title (english)Hepatoprotective effect of polyphenols present in blackthorn extract on antioxidative status in mice
AuthorIvona Paradžik
MentorIrena Landeka Jurčević (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Food Quality Control)
(Laboratory for Food Chemistry and Biochemistry)
Zagreb
Defense date and country2016-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Cilj ovog rada bio je ispitati antioksidativni utjecaj polifenola ekstrakta cvijeta trnine (Prunus spinosa L.) i visoko-proteinske dijete (whey protein) na markere oksidacijskog stresa u jetri C57BL6 miša. Ispitivanje je provedeno kroz 30 dana, pri čemu su životinje bile podijeljene u 4 grupe, ovisno o vrsti tretmana: 1. grupa- kontrolna, 2. grupa- TC (tretirane ekstraktom cvijeta trnine), 3. grupa- WP (tretirane whey proteinom), 4. grupa- WP+TC. Karbonilirani proteini (PC), reducirani glutation (GSH), malondialdehid (MDA), superoksid dismutaza (SOD) te katalaza (CAT) su biomarkeri oksidacijskog stresa praćeni u ovom istraživanju. U odnosu na kontrolnu skupinu životinja, tretman s TC statistički je značajno (ANOVA, p<0,05) smanjio aktivnosti MDA i PC u jetri miša. Takav rezultat pripisuje se prisutnim polifenolima u ekstraktu cvijeta trnine. U odnosu na kontrolnu skupinu životinja, koncentracije SOD, GSH i CAT su u svim grupama životinja statistički značajno (ANOVA, p<0,05) povećane, što znači da se oksidacijski stres smanjio. Takav rezultat posljedica je antioksidacijskog učinka i ekstrakta trnine i whey proteina. Istraživanjem se utvrdilo da ekstrakt cvijeta trnine sadrži mnogo bioaktivnih spojeva koji, u kombinaciji sa primjenom visoko-proteinske dijete (whey protein), imaju povoljan utjecaj na smanjenje oksidacijskog stresa i povećanje aktivnosti endogenih antioksidacijskih enzima.
Abstract (english)
The aim of this study was to examine antioxidant effect of polyphenols from blackthorn (Prunus spinosa L.) extract and high-protein (whey protein) diet on markers of oxidative stress in the liver of C57BL6 mice. Testing obtained for 30 days and the animals were divided into 4 groups, depending on the type of treatment: group 1- control, group 2- TC (treated with blackthorn flower extract), group 3- WP (treated with whey protein) and group 4 - WP+TC. Carbonylated proteins (PC), reduced glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were biomarkers of oxidative stress monitored in this study. Compared to the control group of animals, treatment with TC statistically significant (ANOVA, p<0,05) reduced the activity od MDA and PC in the liver of mice. The result is attributed to the polyphenols present in the extract of a blackthorn flower. Compared to the control group of animals, concentrations of SOD, CAT and GSH in all groups of animals were statistically significant (ANOVA, p<0,05) increased, which means that oxidative stress and it's damage has been reduced. This study found that blackthorn flower extract has many bioactive compounds, which in combination with using high-protein diet (whey protein) have positive effect in reducing oxidative stress and increasing the activity of endogenous antioxidant enzymes.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:490655
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-24 09:10:25