prikaz prve stranice dokumenta Uloga S-layer proteina probiotičkog soja Lactobacillus helveticus M92 u kompetitivnoj ekskluziji bakterije Salmonella enterica subsp. Typhimurium FP1
Access restricted to students and staff of home institution
undergraduate thesis
Uloga S-layer proteina probiotičkog soja Lactobacillus helveticus M92 u kompetitivnoj ekskluziji bakterije Salmonella enterica subsp. Typhimurium FP1
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. urn:nbn:hr:159:243958
Jurišić, Anamarija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Jurišić, A. (2011). Uloga S-layer proteina probiotičkog soja Lactobacillus helveticus M92 u kompetitivnoj ekskluziji bakterije Salmonella enterica subsp. Typhimurium FP1 (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:243958

Jurišić, Anamarija. "Uloga S-layer proteina probiotičkog soja Lactobacillus helveticus M92 u kompetitivnoj ekskluziji bakterije Salmonella enterica subsp. Typhimurium FP1." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:243958

Jurišić, Anamarija. "Uloga S-layer proteina probiotičkog soja Lactobacillus helveticus M92 u kompetitivnoj ekskluziji bakterije Salmonella enterica subsp. Typhimurium FP1." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:243958

Jurišić, A. (2011). 'Uloga S-layer proteina probiotičkog soja Lactobacillus helveticus M92 u kompetitivnoj ekskluziji bakterije Salmonella enterica subsp. Typhimurium FP1', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 05 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:243958

Jurišić A. Uloga S-layer proteina probiotičkog soja Lactobacillus helveticus M92 u kompetitivnoj ekskluziji bakterije Salmonella enterica subsp. Typhimurium FP1 [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2011 [cited 2024 November 05] Available at: https://urn.nsk.hr/urn:nbn:hr:159:243958

A. Jurišić, "Uloga S-layer proteina probiotičkog soja Lactobacillus helveticus M92 u kompetitivnoj ekskluziji bakterije Salmonella enterica subsp. Typhimurium FP1", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2011. Available at: https://urn.nsk.hr/urn:nbn:hr:159:243958

Metadata
TitleUloga S-layer proteina probiotičkog soja Lactobacillus helveticus M92 u kompetitivnoj ekskluziji bakterije Salmonella enterica subsp. Typhimurium FP1
Title (english)Functionality of the S-layer protein from the probiotic strain Lactobacillus helveticus M92 in the competitive exclusion of Salmonella enterica subsp. Typhimurium FP1
AuthorAnamarija Jurišić
MentorJasna Novak (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2011-06-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj ovog istraživanja je bio ispitati funkcionalnu ulogu S-layer proteina (SlpA) probiotičkog soja Lactobacillus helveticus M92 u kompetitivnoj ekskluziji Salmonella enterica subsp. Typhimurium FP1. Prethodna istraživanja upućuju na mogućnost smanjenja infekcije bakterijom S. Typhimurium FP1, kod eksperimentalnih miševa, nakon oralne primjene probiotičkog soja L. helveticus M92, što može biti posljedica kompetitivne ekskluzije ovog soja u gastrointestinalnom traktu (GIT). U ovom radu je ispitan mikrobni antagonizam probiotičkog soja L. helveticus M92 prema S. Typhimurium FP1, te je ustanovljeno da L. helveticus M92 inhibira rast S. Typhimurium FP1 tijekom 24 sata uzgoja. Stoga je ispitana i koagregacija ovog probiotičkog soja sa S. Typhimurium FP1. Provedena su i istraživanja adhezije L. helveticus M92 na Caco-2 epitelne stanice. Ispitana je i mogućnost smanjenja infekcije Caco-2 epitelnih stanica sa S. Typhimurium FP1 nakon primjene probiotičkog soja L. helveticus M92, te uloga S-layer proteina. Preliminarni rezultati pokazali su da primjena probiotičkog soja L. helveticus M92 inhibira invaziju patogene bakterije S. Typhimurium FP1 na Caco-2 epitelne stanice.
Abstract (english)
The objective of this study was to investigate the functional role of the S-layer protein (SlpA) from probiotic strain Lactobacillus helveticus M92 in the competitive exclusion of Salmonella enterica subsp. Typhimurium FP1. The previous researches confirmed that after the oral treatment of experimental mice, infected with S. Typhimurium FP1, with the probiotic strain L. helveticus M92, the pathogen load of Salmonella was lower, as a result of the competitive exclusion of this strain in intestinal tract conferred by probiotic. In this study, microbial antagonism of L. helveticus M92 against S. Typhimurium FP1 was investigated. L. helveticus M92 reduced the growth of S. Typhimurium FP1 during the 24 hours. Therefore, the coaggregation of S. Typhimurium FP1 with L. helveticus M92 was investigated as well. Also, investigation of the adhesion ability of L. helveticus M92 cells on Caco-2 epithelium cells, as well as the possibility to decrease the infection of the Caco-2 cells with S. Typhimurium FP1 after treatment of probiotic strain L. helveticus M92 and the role of S-layer protein in this mechanism were investigated. Preliminary results have shown that application of L. helveticus M92 inhibits in vitro invasion of the S. Typhimurium FP1cells on Caco-2 epihtelium cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:243958
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-18 12:29:18