prikaz prve stranice dokumenta Određivanje optimalnih uvjeta aktivnosti proteina YblCcw12p eksprimiranog u kvascu Saccharomyces cerevisiae
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undergraduate thesis
Određivanje optimalnih uvjeta aktivnosti proteina YblCcw12p eksprimiranog u kvascu Saccharomyces cerevisiae
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. urn:nbn:hr:159:452223
Didak, Blanka
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Didak, B. (2011). Određivanje optimalnih uvjeta aktivnosti proteina YblCcw12p eksprimiranog u kvascu Saccharomyces cerevisiae (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:452223

Didak, Blanka. "Određivanje optimalnih uvjeta aktivnosti proteina YblCcw12p eksprimiranog u kvascu Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:452223

Didak, Blanka. "Određivanje optimalnih uvjeta aktivnosti proteina YblCcw12p eksprimiranog u kvascu Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:452223

Didak, B. (2011). 'Određivanje optimalnih uvjeta aktivnosti proteina YblCcw12p eksprimiranog u kvascu Saccharomyces cerevisiae', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 30 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:452223

Didak B. Određivanje optimalnih uvjeta aktivnosti proteina YblCcw12p eksprimiranog u kvascu Saccharomyces cerevisiae [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2011 [cited 2024 December 30] Available at: https://urn.nsk.hr/urn:nbn:hr:159:452223

B. Didak, "Određivanje optimalnih uvjeta aktivnosti proteina YblCcw12p eksprimiranog u kvascu Saccharomyces cerevisiae", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2011. Available at: https://urn.nsk.hr/urn:nbn:hr:159:452223

Metadata
TitleOdređivanje optimalnih uvjeta aktivnosti proteina YblCcw12p eksprimiranog u kvascu Saccharomyces cerevisiae
Title (english)Determination of optimal conditions for activity of protein YblCcw12p expressed in yeast Saccharomyces cerevisiae
AuthorBlanka Didak
MentorRenata Teparić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2011-06-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U posljednje vrijeme raste interes za biotehnološku primjenu enzima imobiliziranih na površinu stanica domaćina koja tada ujedno djeluje kao producent i nosač imobiliziranog enzima. Od posebne važnosti su heterologni proteini vezani na staničnu stijenku kvasca Saccharomyces cerevisiae, budući da je kvasac nepatogeni mikroorganizam koji se lako transformira i uzgaja, a kao eukariot je pogodniji za ekspresiju enzima sisavaca nego bakterijske stanice. U ovom radu je karakterizirana enzimska aktivnost rekombinantne nukleaze YblCcw12p koja je dobijena fuzijom gena za intracelularnu nukleazu kvasca Yblp i gena za protein stanične stijenke Ccw12p preko kojeg je onda rekombinantni protein usidren u stijenku. U radu je pokazano da je rekombinantni enzim uspješno imobiliziran na površini stanice i da ima nukleaznu aktivnost. Za detekciju enzimske aktivnosti korištene su elektroforeza i SRED metoda koje su detaljno opisane u radu. Nadalje je provedena optimizacija metode za mjerenje enzimske aktivnosti modificiranjem metode po Kunitz-u, te su određeni optimalni uvijeti za aktivnost rekombinantnog enzima.
Abstract (english)
In the past decade interest is growing for the display of heterologous proteins on the surface of microorganisms, serving at the same time as producers and carriers of immobilized enzymes. Saccharomyces cerevisiae, as nonpathogenic microorganism which is easily transformed, cultivated, and better host for expression of mammalian enzymes than bacterial cells, has special importance in this field of biotechnology. In this work enzyme activity of recombinant nuclease YblCcw12p is detected and partially characterized. YblCcw12p is made by fusion of gene for yeast intracellular nuclease Yblp and gene for cell wall protein Ccw12p. Results presented in this work showed that recombinant enzyme is successfully immobilized on surface of the cell and posses nuclease activity. Electrophoresis and SRED method were used for detection of enzyme activity. Furthermore, enzyme activity was measured and optimal conditions for activity of recombinant protein were determined by modified and optimized Kunitz method.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:452223
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-19 08:00:23