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master's thesis
Utjecaj sastava podloge i uvjeta okoline na proizvodnju (S)-adenozil-L-metionina pomoću kvasca Pichia stipitis
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. urn:nbn:hr:159:873851
Percela, Ana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Food Engineering
Laboratory for Fermentation and Yeast Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Percela, A. (2011). Utjecaj sastava podloge i uvjeta okoline na proizvodnju (S)-adenozil-L-metionina pomoću kvasca Pichia stipitis (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:873851

Percela, Ana. "Utjecaj sastava podloge i uvjeta okoline na proizvodnju (S)-adenozil-L-metionina pomoću kvasca Pichia stipitis." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:873851

Percela, Ana. "Utjecaj sastava podloge i uvjeta okoline na proizvodnju (S)-adenozil-L-metionina pomoću kvasca Pichia stipitis." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:873851

Percela, A. (2011). 'Utjecaj sastava podloge i uvjeta okoline na proizvodnju (S)-adenozil-L-metionina pomoću kvasca Pichia stipitis', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 23 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:873851

Percela A. Utjecaj sastava podloge i uvjeta okoline na proizvodnju (S)-adenozil-L-metionina pomoću kvasca Pichia stipitis [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2011 [cited 2024 June 23] Available at: https://urn.nsk.hr/urn:nbn:hr:159:873851

A. Percela, "Utjecaj sastava podloge i uvjeta okoline na proizvodnju (S)-adenozil-L-metionina pomoću kvasca Pichia stipitis", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2011. Available at: https://urn.nsk.hr/urn:nbn:hr:159:873851

Metadata
TitleUtjecaj sastava podloge i uvjeta okoline na proizvodnju (S)-adenozil-L-metionina pomoću kvasca Pichia stipitis
Title (english)Influence of medium composition and environmental conditions on the production of S-adenosyl-L-methionine with the yeast Pichia stipitis
AuthorAna Percela
MentorDamir Stanzer (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Food Engineering)
(Laboratory for Fermentation and Yeast Technology)
Zagreb
Defense date and country2011-04-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
(S)-adenozil-L-metionin (SAMe) je molekula važna za život svake stanice, jer je univerzalni donor metilne skupine. Nastaje sintezom iz ATP-a i metionina djelovanjem metionin-adenoziltransferaze. Sudjeluje u tri važna metabolička puta: transmetilacija, trans-sulfuracija te biosinteza poliamina. Nedostatak SAMe u ljudskom organizmu kao i poremećaji u metabolizmu SAMe mogu se manifestirati kroz različite poremećaje u funkcioniranju jetre, te imunološkom i živčanom sustavu. Cilj ovog rada bio je proučiti utjecaj sastava podloge i uvjeta okoline na proizvodnju SAMe pomoću kvasca Pichia stipitis pri uzgoju na laboratorijskoj tresilici. Rezultati pokazuju da se najveća količina akumuliranog SAMe ostvaruje u 18-om satu uzgoja (69,63 mg/g s.tv.kvasca) u glukoznoj podlozi s dodatkom 4,5 g/L D,L-metionina, pri temperaturi od 30oC i pH vrijednosti 4,8.
Abstract (english)
S-Adenosyl-L-methionine (SAMe) is important for all living cells, because it is universal methyl donor. This substance is synthesized from adenosine triphosphate (ATP) and methionine by methionine adenosyltransferase. SAMe participates in three important metabolic pathways: transmethylation, transsulfuration and the polyamine biosynthesis. Deficiency of SAMe in human organism as well as abnormalities in SAMe metabolism can be manifested through different disfunctions in functioning of a liver, immunological and nervous system. The aim of this work was determine the influence of medium composition and environmental conditions on the production of SAMe in the yeast Pichia stipitis, grown at laboratory shaker. The results showed that Pichia stipitis reaches maximal SAMe accumulation in 18th hour of cultivation (69.63 mg/g dry cells Yeast) in glucose media with the addition of 4.5 g/L D,L-methionine, at a temperature of 30° C and pH value 4.8.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:873851
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-30 10:43:50