prikaz prve stranice dokumenta Production and characterization of mouse monoclonal antibodies against human and mouse ribosomal proteins L5 and L11
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master's thesis
Production and characterization of mouse monoclonal antibodies against human and mouse ribosomal proteins L5 and L11
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. urn:nbn:hr:159:711272
Jelenčić, Vedrana
University of Zagreb
Faculty of Food Technology and Biotechnology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Jelenčić, V. (2010). Production and characterization of mouse monoclonal antibodies against human and mouse ribosomal proteins L5 and L11 (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:711272

Jelenčić, Vedrana. "Production and characterization of mouse monoclonal antibodies against human and mouse ribosomal proteins L5 and L11." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. https://urn.nsk.hr/urn:nbn:hr:159:711272

Jelenčić, Vedrana. "Production and characterization of mouse monoclonal antibodies against human and mouse ribosomal proteins L5 and L11." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. https://urn.nsk.hr/urn:nbn:hr:159:711272

Jelenčić, V. (2010). 'Production and characterization of mouse monoclonal antibodies against human and mouse ribosomal proteins L5 and L11', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 08 October 2024, https://urn.nsk.hr/urn:nbn:hr:159:711272

Jelenčić V. Production and characterization of mouse monoclonal antibodies against human and mouse ribosomal proteins L5 and L11 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2010 [cited 2024 October 08] Available at: https://urn.nsk.hr/urn:nbn:hr:159:711272

V. Jelenčić, "Production and characterization of mouse monoclonal antibodies against human and mouse ribosomal proteins L5 and L11", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2010. Available at: https://urn.nsk.hr/urn:nbn:hr:159:711272

Metadata
TitleProduction and characterization of mouse monoclonal antibodies against human and mouse ribosomal proteins L5 and L11
AuthorVedrana Jelenčić
MentorLidija Šver (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
Zagreb
Defense date and country2010-07-02, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
The mammalian ribosome comprises 80 ribosomal proteins. Ribosomal proteins L5 and L11 are essential components of ribosome and recent evidence demonstrates that they participate in p53 tumor suppressor activation by interacting with Mdm2 and to bind to and influence activity of growth regulating transcription factor c-Myc. Due to lack of specific monoclonal antibodies against RpL5 and RpL11, which are required for further study of these and possible other extraribosomal functions of RpL11 and RpL5, we decided to produce mouse monoclonal antibodies against these ribosomal proteins. Ribosomal proteins are almost identical in mammals and therefore it is difficult to produce antibodies against them. Here we describe production of monoclonal antibodies directed against mouse and human RpL5 and RpL11 by immunizing mice with recombinant mouse Rps isolated from bacteria. Immune response against self-antigens in mice was successfully elicited by injecting large amounts of antigens combined with Freund's adjuvant. All produced mouse antibodies, including one against RpL11 and three against RpL5, could specifically recognize corresponding recombinant Rp on ELISA. While one monoclonal antibody against RpL5 could specifically recognize endogenous RpL5 in western blot analysis, other produced monoclonal antibodies require further testing. Beside to production of valuable antibodies for further characterization of RpL11 and RpL5 functions, described protocol show in general how to produce mouse monoclonal antibodies against highly conserved and thus less immunogenic antigens.
Keywords
Languageenglish
URN:NBNurn:nbn:hr:159:711272
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-06-02 11:38:10