prikaz prve stranice dokumenta Production and characterization of mouse monoclonal antibodies against human and mouse ribosomal proteins L5 and L11
Pristup korisnicima matične ustanove
diplomski rad
Production and characterization of mouse monoclonal antibodies against human and mouse ribosomal proteins L5 and L11
Zagreb: Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet, 2010. urn:nbn:hr:159:711272
Jelenčić, Vedrana
Sveučilište u Zagrebu
Prehrambeno-biotehnološki fakultet

Institucijski repozitorij: Repozitorij Prehrambeno-biotehnološkog fakulteta u Zagrebu

Citirajte ovaj rad

Jelenčić, V. (2010). Production and characterization of mouse monoclonal antibodies against human and mouse ribosomal proteins L5 and L11 (Diplomski rad). Zagreb: Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet. Preuzeto s https://urn.nsk.hr/urn:nbn:hr:159:711272

Jelenčić, Vedrana. "Production and characterization of mouse monoclonal antibodies against human and mouse ribosomal proteins L5 and L11." Diplomski rad, Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet, 2010. https://urn.nsk.hr/urn:nbn:hr:159:711272

Jelenčić, Vedrana. "Production and characterization of mouse monoclonal antibodies against human and mouse ribosomal proteins L5 and L11." Diplomski rad, Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet, 2010. https://urn.nsk.hr/urn:nbn:hr:159:711272

Jelenčić, V. (2010). 'Production and characterization of mouse monoclonal antibodies against human and mouse ribosomal proteins L5 and L11', Diplomski rad, Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet, citirano: 25.11.2024., https://urn.nsk.hr/urn:nbn:hr:159:711272

Jelenčić V. Production and characterization of mouse monoclonal antibodies against human and mouse ribosomal proteins L5 and L11 [Diplomski rad]. Zagreb: Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet; 2010 [pristupljeno 25.11.2024.] Dostupno na: https://urn.nsk.hr/urn:nbn:hr:159:711272

V. Jelenčić, "Production and characterization of mouse monoclonal antibodies against human and mouse ribosomal proteins L5 and L11", Diplomski rad, Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet, Zagreb, 2010. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:159:711272

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:159:711272
Podaci o radu
NaslovProduction and characterization of mouse monoclonal antibodies against human and mouse ribosomal proteins L5 and L11
AutorVedrana Jelenčić
MentorLidija Šver (mentor)
Ustanova koja je dodijelila
akademski / stručni stupanj		Sveučilište u Zagrebu
Prehrambeno-biotehnološki fakultet
Zagreb
Datum i država obrane2010-07-02, Hrvatska
Znanstveno / umjetničko
područje, polje i grana		BIOTEHNIČKE ZNANOSTI
Biotehnologija
Sažetak
The mammalian ribosome comprises 80 ribosomal proteins. Ribosomal proteins L5 and L11 are essential components of ribosome and recent evidence demonstrates that they participate in p53 tumor suppressor activation by interacting with Mdm2 and to bind to and influence activity of growth regulating transcription factor c-Myc. Due to lack of specific monoclonal antibodies against RpL5 and RpL11, which are required for further study of these and possible other extraribosomal functions of RpL11 and RpL5, we decided to produce mouse monoclonal antibodies against these ribosomal proteins. Ribosomal proteins are almost identical in mammals and therefore it is difficult to produce antibodies against them. Here we describe production of monoclonal antibodies directed against mouse and human RpL5 and RpL11 by immunizing mice with recombinant mouse Rps isolated from bacteria. Immune response against self-antigens in mice was successfully elicited by injecting large amounts of antigens combined with Freund's adjuvant. All produced mouse antibodies, including one against RpL11 and three against RpL5, could specifically recognize corresponding recombinant Rp on ELISA. While one monoclonal antibody against RpL5 could specifically recognize endogenous RpL5 in western blot analysis, other produced monoclonal antibodies require further testing. Beside to production of valuable antibodies for further characterization of RpL11 and RpL5 functions, described protocol show in general how to produce mouse monoclonal antibodies against highly conserved and thus less immunogenic antigens.
Ključne riječi
Jezikengleski
URN:NBNurn:nbn:hr:159:711272
Studijski programNaziv: Molekularna biotehnologija
Vrsta studija: sveučilišni
Stupanj studija: diplomski
Akademski / stručni naziv: magistar/magistra inženjer/inženjerka molekularne biotehnologije (mag. ing. biotechn.)
Vrsta resursaTekst
Način izrade datotekeIzvorno digitalna
Prava pristupaPristup korisnicima matične ustanove
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2017-06-02 11:38:10