prikaz prve stranice dokumenta Factors influencing the success of microspore and anther culture for wheat and triticale haploid production
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master's thesis
Factors influencing the success of microspore and anther culture for wheat and triticale haploid production
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. urn:nbn:hr:159:345655
Brizić, Ilija
University of Zagreb
Faculty of Food Technology and Biotechnology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Brizić, I. (2010). Factors influencing the success of microspore and anther culture for wheat and triticale haploid production (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:345655

Brizić, Ilija. "Factors influencing the success of microspore and anther culture for wheat and triticale haploid production." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. https://urn.nsk.hr/urn:nbn:hr:159:345655

Brizić, Ilija. "Factors influencing the success of microspore and anther culture for wheat and triticale haploid production." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. https://urn.nsk.hr/urn:nbn:hr:159:345655

Brizić, I. (2010). 'Factors influencing the success of microspore and anther culture for wheat and triticale haploid production', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:345655

Brizić I. Factors influencing the success of microspore and anther culture for wheat and triticale haploid production [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2010 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:159:345655

I. Brizić, "Factors influencing the success of microspore and anther culture for wheat and triticale haploid production", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2010. Available at: https://urn.nsk.hr/urn:nbn:hr:159:345655

Metadata
TitleFactors influencing the success of microspore and anther culture for wheat and triticale haploid production
AuthorIlija Brizić
MentorBiljana Balen (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
Zagreb
Defense date and country2010-07-02, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Anther culture technique is employed in laboratory 'Le Menhir' for production of triticale and wheat haploid plants. Microspore culture technique shows a potential to be better than anther culture in haploid production; therefore, it is evaluated in laboratory 'Le Menhir'. This study is one of the steps towards the development of successful microspore culture protocol as well as the part of an evaluation process. In this work, no haploid plants were produced using microspore culture protocol; although microspores from more than 400 spikes were put in culture. This is probably due to the bad health state of donor plants and genetic effects. The haploids were produced using anther culture protocol. The average production of triticale haploids is 1.82 green plants per spike and wheat haploid 0.33 green plants per spike. Genotypic effects on production success are obvious and they are presented in the work. Furthermore, the parent genotypes are classified by the average success rates of their progeny, which genotype’s 15580 progeny has the best average success rate. This data will be used in future experiments. In addition, the development of microspores towards embryos is described. As the next step verification of these results is proposed as well as modifications of the microspore culture protocol.
Keywords
Languageenglish
URN:NBNurn:nbn:hr:159:345655
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-06-02 13:03:21