prikaz prve stranice dokumenta Biokemijska i fizikalna analiza stanične stijenke kvasca
  Download
PDF 1.68 MB
master's thesis
Biokemijska i fizikalna analiza stanične stijenke kvasca
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. urn:nbn:hr:159:788910
Zeljko, Danijela
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Zeljko, D. (2016). Biokemijska i fizikalna analiza stanične stijenke kvasca (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:788910

Zeljko, Danijela. "Biokemijska i fizikalna analiza stanične stijenke kvasca." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:788910

Zeljko, Danijela. "Biokemijska i fizikalna analiza stanične stijenke kvasca." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:788910

Zeljko, D. (2016). 'Biokemijska i fizikalna analiza stanične stijenke kvasca', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 30 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:788910

Zeljko D. Biokemijska i fizikalna analiza stanične stijenke kvasca [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2016 [cited 2024 November 30] Available at: https://urn.nsk.hr/urn:nbn:hr:159:788910

D. Zeljko, "Biokemijska i fizikalna analiza stanične stijenke kvasca", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:159:788910

Metadata
TitleBiokemijska i fizikalna analiza stanične stijenke kvasca
Title (english)Biochemical and biophysics studies of cell wall of industrial yeasts
AuthorDanijela Zeljko
MentorVladimir Mrša (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2016-10-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj ovog rada je bio odrediti udio komponenti stanične stijenke kvasca Saccharomyces cerevisiae: manoproteina, β-1,3-glukana, β-1,6 glukana i hitina, koristeći novo razvijenu metodu koja se temelji na enzimskim i kemijskim metodama. Ova metoda je testirana na različitim sojevima kvasca i došlo se do zaključka da uvjeti rasta imaju utjecaj na udio staničnih komponenti. Metoda je korištena za procjenu sastava stanične stijenke industrijskih sojeva (autolizirani i osušeni sojevi) koji su bili pod utjecajem različitih fermentacijskih i drugih procesa. Korišteni su i fenotipski testovi da se uoči i potvrdi da rast različitih sojeva ovisi o sastavu hranjive podloge. U drugom dijelu rada je promatran utjecaj etanol stresa na rast te na udio komponenti stanične stijenke različitih laboratorijskih sojeva kvasca.
Abstract (english)
The aim of this study was to quantify the amount of specific components in the cell wall of yeast Saccharomyces cerevisiae, namely mannoproteins, β-1,3-glucans and β-1,6-glucans and chitin using newly developed enzymatic and chemical method. These new methods were validated on various cell wall mutants and it was observed that growth conditions have strong effects on the amount of cell wall components. This method was used to evaluate cell wall composition of industrial yeasts (autolysed and dried yeast) that were subjected to different fermentation and treatment processes. The phenotypic assay was used to confirm and to notice how the growth of various strains depends on composition of medium. The second part of the project was to examine the effect of ethanol stress on cell wall composition and to analyze cell wall composition in industrial yeast cells, to determine whether culture/process conditions have an impact on cell wall composition.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:159:788910
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-25 11:12:01