prikaz prve stranice dokumenta Primjena proteomike i drugih molekularnih metoda u definiranju funkcionalnih svojstava probiotičkih bakterija
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doctoral thesis
Primjena proteomike i drugih molekularnih metoda u definiranju funkcionalnih svojstava probiotičkih bakterija
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2008. urn:nbn:hr:159:210428
Beganović, Jasna
University of Zagreb
Faculty of Food Technology and Biotechnology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Beganović, J. (2008). Primjena proteomike i drugih molekularnih metoda u definiranju funkcionalnih svojstava probiotičkih bakterija (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:210428

Beganović, Jasna. "Primjena proteomike i drugih molekularnih metoda u definiranju funkcionalnih svojstava probiotičkih bakterija." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2008. https://urn.nsk.hr/urn:nbn:hr:159:210428

Beganović, Jasna. "Primjena proteomike i drugih molekularnih metoda u definiranju funkcionalnih svojstava probiotičkih bakterija." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2008. https://urn.nsk.hr/urn:nbn:hr:159:210428

Beganović, J. (2008). 'Primjena proteomike i drugih molekularnih metoda u definiranju funkcionalnih svojstava probiotičkih bakterija', Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 05 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:210428

Beganović J. Primjena proteomike i drugih molekularnih metoda u definiranju funkcionalnih svojstava probiotičkih bakterija [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2008 [cited 2024 November 05] Available at: https://urn.nsk.hr/urn:nbn:hr:159:210428

J. Beganović, "Primjena proteomike i drugih molekularnih metoda u definiranju funkcionalnih svojstava probiotičkih bakterija", Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2008. Available at: https://urn.nsk.hr/urn:nbn:hr:159:210428

Metadata
TitlePrimjena proteomike i drugih molekularnih metoda u definiranju funkcionalnih svojstava probiotičkih bakterija
Title (english)Application of proteomics and other molecular methods in characterisation of functionality of the probiotic bacteria
AuthorJasna Beganović
MBZ: 267973
MentorJagoda Šušković (mentor)
MBZ: 40116
Committee memberVladimir Mrša (predsjednik povjerenstva)
MBZ: 39525
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
Zagreb
Defense date and country2008-07-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Universal decimal classification
(UDC)		60 - Biotechnology. Genetic engineering. Products of cloning
Abstract
Cilj ovog rada je definiranje funkcionalnih svojstava dviju bakterija mliječne kiseline na molekularnoj razini: bakterije Lactococcus lactis koja je modelni mikroorganizam za proučavanje fiziologije BMK i probiotičke bakterije Lactobacillus acidophilus M92. U tu svrhu primjenjene su komplementarne molekularne metode, u prvom redu proteomičke. Razvijeni postupci su omogućili identifikaciju 490 proteina netopljivog proteoma koji je nadopunjuje citoplazmatski proteom bakterije L. lactis. Zbog mogućnost primjene L. lactis kao živog prijenosnika vakcina ili heterolognih proteina, analiziran je i netopljivi proteom ove bakterije nakon prolaska kroz gastrointestinalni sustav miša. Statistička analiza rezultata omogućila je usporedbu proteoma bakterije L. lactis iz dvaju različitih mikrookoliša, te identifikaciju proteinskih markera adaptacije na stresne uvjete gastrointestinalnog trakta. Proteomički pristup primjenjen za L. lactis, također se primjenio za analizu proteoma probiotičkog soja L. acidophilus M92 s posebnim osvrtom na SlpA površinske proteine koji imaju nekoliko različitih primjena, uključujući njihovu uporabu kao nositelja vakcina.
Abstract (english)
The aim of this research was a characterisation of functional properties of two lactic acid bacteria (LAB) on a molecular level: Lactococcus lactis, which is a model bacterium for studying LAB physiology and probiotic strain Lactobacillus acidophilus M92. Therefore, complementary molecular methods were applied and prior proteomics. Developed approaches provided identification of 490 different proteins of an insoluble L. lactis proteome. The analysis of L. lactis insoluble proteome after the passage of this bacterium through the gastrointestinal tract (GIT) of mice was undertaken as there is a potential of L. lactis use as live oral vector for vaccine and heterologous proteins. Statistical analysis enabled comparison L. lactis proteomes from two very different environments and identification of protein biomarkers of adaptation of this bacterium to GIT. The proteomic approach developed on L. lactis, was applied for the proteome analysis of probiotic strain L. acidophilus M92 with a special focus on SlpA surface proteins which already have a few applications including their use as an antigen delivery vehicles.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:210428
Promotion2009-09-13
Study programmeTitle: Postgraduate PhD Study in Biotechnology and Bioprocess Engineering
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2018-05-14 08:56:57