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doctoral thesis
Mehanizmi ugradnje proteina Scw4 i Scw10 u staničnu stijenku kvasca Saccharomyces cerevisiae i njihova fiziološka uloga
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:819933
Grbavac, Antonija
University of Zagreb
Faculty of Food Technology and Biotechnology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Grbavac, A. (2017). Mehanizmi ugradnje proteina Scw4 i Scw10 u staničnu stijenku kvasca Saccharomyces cerevisiae i njihova fiziološka uloga (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:819933

Grbavac, Antonija. "Mehanizmi ugradnje proteina Scw4 i Scw10 u staničnu stijenku kvasca Saccharomyces cerevisiae i njihova fiziološka uloga." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:819933

Grbavac, Antonija. "Mehanizmi ugradnje proteina Scw4 i Scw10 u staničnu stijenku kvasca Saccharomyces cerevisiae i njihova fiziološka uloga." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:819933

Grbavac, A. (2017). 'Mehanizmi ugradnje proteina Scw4 i Scw10 u staničnu stijenku kvasca Saccharomyces cerevisiae i njihova fiziološka uloga', Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:819933

Grbavac A. Mehanizmi ugradnje proteina Scw4 i Scw10 u staničnu stijenku kvasca Saccharomyces cerevisiae i njihova fiziološka uloga [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:159:819933

A. Grbavac, "Mehanizmi ugradnje proteina Scw4 i Scw10 u staničnu stijenku kvasca Saccharomyces cerevisiae i njihova fiziološka uloga", Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:819933

Metadata
TitleMehanizmi ugradnje proteina Scw4 i Scw10 u staničnu stijenku kvasca Saccharomyces cerevisiae i njihova fiziološka uloga
Title (english)Mechanism of incorporation of Scw4 and Scw10 proteins in the Saccharomyces cerevisiae cell wall and their physiological role
AuthorAntonija Grbavac
MentorVladimir Mrša (mentor)
Committee memberRenata Teparić (predsjednik povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
Zagreb
Defense date and country2017-04-18, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Universal decimal classification
(UDC)		54 - Chemistry. Crystallography. Mineralogy
Abstract
Stanična stijenka kvasca Saccharomyces cerevisiae građena je od glukana, hitina, te najmanje 30 različitih manoproteina. U fokusu ovog istraživanja bila su dva proteina lokalizirana u staničnoj stijenci kvasca, Scw4 i njegov strukturni homolog Scw10, koji su prvo opisani kao proteini koji sa strukturama u stijenci ostvaruju nekovalentne interakcije. Ipak, kasnije je utvrđeno da se dio Scw4 na stijenku veže kovalentnom vezom, dok se Scw10 i dalje smatra proteinom koji je vezan isključivo nekovalentnim interakcijama. Analize sekvenci Scw4 i Scw10 pokazale su kako oba proteina sadrže motive koji se smatraju paradigmatskim mjestima procesiranja Kex2. Fiziološka uloga ovih proteina nije poznata, no utvrđeno je da simultana disrupcija gena SCW4 i SCW10 znatno povećava udio mrtvih stanica, te rezultira nemogućnošću parenja mutiranih sojeva. U sklopu izrade disertacije utvrđeno je da Scw4 podliježe procesiranju japsinima koji cijepaju ovaj supstrat nizvodno od motiva koji prepoznaje Kex2, te da na ovaj način generirana forma proteina ima smanjenu efikasnost kovalentnog vezanja na stijenku. Prisutnost povećane količine potpuno procesirane forme Scw4 smanjuje vijabilnost soja, te uzrokuje značajno povećanje volumena kod određenog broja stanica. S druge strane prisutnost povećane količine neprocesirane forme proteina, kao i one koja je procesirana s Kex2, ne uzrokuje promjene fenotipa što upućuje na zaključak kako je jedino potpuno procesirana forma Scw4 aktivna. Također, utvrđeno je da Scw10 biva procesiran samo na jednom mjestu, u reakciji koju katalizira Kex2, no moguće je da u odsutnosti ove proteaze Scw10 postaje supstrat japsina koji ovaj protein cijepaju na istoj poziciji.
Abstract (english)
Saccharomyces cerevisiae cell wall is composed of glucan, chitin and at least 30 different manoproteins. This research was focused on two proteins located in yeast cell wall, Scw4 and its structural homolog Scw10, that were first identified as proteins non-covalently bound to the structures in wall. It was later shown that a part of Scw4 was also covalently attached to wall, while Scw10 is still considered non-covalently bound protein. It was observed that both Scw4 and Scw10 contain paradigmatic Kex2 processing site. Physiological role of these proteins is still unknown, however it was shown that simultaneous disruption of genes SCW4 and SCW10 causes increased mortality of the strain and inability to mate. Here we show that Scw4 is being processed by yapsins at a position located downstream of the Kex2 cleavage site. Scw4 cleaved at the yapsin site had a lower potential for covalent attachment to glucan. The overproduction of the fully processed form of Scw4 lead to high mortality of the strain and markedly increased size of portion of cells, while the overproduction of Scw4 processed only by Kex2, or not processed one, caused no apparent change in mortality of morphology. This indicates that only the smallest, completely mature form of Scw4 had the activity leading to observed phenotype changes. It was also shown that Scw10 is being cleaved only at one position, by Kex2, however it was observed that in absence of this protease, part of Scw10 is still being processed at the same site, possibly by a member of yapsine family.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:819933
Study programmeTitle: Postgraduate PhD Study in Biotechnology and Bioprocess Engineering
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2018-05-18 11:50:43