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undergraduate thesis
Enantioselektivna hidroliza (R, S)-1-feniletil- acetata u prirodnim eutektičkim otapalima primjenom lipaze kao biokatalizatora
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:718596
Štokić, Marina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Štokić, M. (2018). Enantioselektivna hidroliza (R, S)-1-feniletil- acetata u prirodnim eutektičkim otapalima primjenom lipaze kao biokatalizatora (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:718596

Štokić, Marina. "Enantioselektivna hidroliza (R, S)-1-feniletil- acetata u prirodnim eutektičkim otapalima primjenom lipaze kao biokatalizatora." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:718596

Štokić, Marina. "Enantioselektivna hidroliza (R, S)-1-feniletil- acetata u prirodnim eutektičkim otapalima primjenom lipaze kao biokatalizatora." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:718596

Štokić, M. (2018). 'Enantioselektivna hidroliza (R, S)-1-feniletil- acetata u prirodnim eutektičkim otapalima primjenom lipaze kao biokatalizatora', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 30 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:718596

Štokić M. Enantioselektivna hidroliza (R, S)-1-feniletil- acetata u prirodnim eutektičkim otapalima primjenom lipaze kao biokatalizatora [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 November 30] Available at: https://urn.nsk.hr/urn:nbn:hr:159:718596

M. Štokić, "Enantioselektivna hidroliza (R, S)-1-feniletil- acetata u prirodnim eutektičkim otapalima primjenom lipaze kao biokatalizatora", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:718596

Metadata
TitleEnantioselektivna hidroliza (R, S)-1-feniletil- acetata u prirodnim eutektičkim otapalima primjenom lipaze kao biokatalizatora
Title (english)Enantioselective hydrolysis of (R, S)-1-phenylethyl acetate in natural deep eutectic solvents with application of lipase as biocatalyst
AuthorMarina Štokić
MentorMarina Cvjetko Bubalo (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2018-09-10, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Implementacija pristupa zelene kemije u posljednje vrijeme postaje važan cilj mnogih industrija u proizvodnji kiralnih građevnih blokova, a razlog je štetni utjecaj dosad primjenjivanih metoda sinteze na ljudsko zdravlje i okoliš. Zelena otapala, posebice prirodna eutektička otapala, zelena su zamjena za dosada korištena štetna i hlapljiva otapala. Biokataliza kao primjena bioloških sustava je zeleniji način sinteze sa mogućnošću smanjenja otpada i energetskog troška. U ovom radu ispituje se mogućnost korištenja prirodnih eutektičkih otapala na bazi kolin-klorida u enantioselektivnoj hidrolizi (R, S)-1-feniletil-acetata, uz lipazu kao biokatalizator. Hidroliza je uspješno provedena u svim pripremljenim prirodnim eutektičkim otapalima, od kojih se kao najučinkovitije otapalo pokazalo kolin-klorid:glukoza s 50 % (v/v) vode (η=88,6 %, ee=98 % ). Također, ispitan je i utjecaj ultrazvuka kao alternativnog izvora energije na prinos reakcije hidrolize, te je utjecaj bio pozitivan: vrijeme reakcije se skratilo, a produktivnost povećala.
Abstract (english)
The implementation of green chemistry approach in industry for production of chiral building blocks has lately developed, with the main reason being bad impact of conventional synthesis on human health and environment. Natural deep eutectic solvents, as green solvents, are a green substitute for toxic and volatile solvents. Biocatalysis, as application of biological systems, represents a greener way of synthesis with reduction of waste and energetic expense. In this study, the application of choline-chloride based natural deep eutectic solvents in enantioselective hydrolysis of (R, S)-1-phenyletyl-acetate using lipase as biocatalyst will be examined. The hydrolysis was successfully carried out in all natural deep eutectic solvents, the most effective was choline-chloride: glucose solvent with 50%(v/v) of water (η =88,6%, ee =98%). Also, the impact of ultrasound as alternative energy source was examined, the results were positive and manifested in decrease of reaction time and productivity magnification.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:718596
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2018-09-14 07:34:37