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master's thesis
Karakterizacija i kultivacija knock-out sojeva bakterije Escherichia coli
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:750958
Brkan, Katarina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Brkan, K. (2017). Karakterizacija i kultivacija knock-out sojeva bakterije Escherichia coli (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:750958

Brkan, Katarina. "Karakterizacija i kultivacija knock-out sojeva bakterije Escherichia coli." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:750958

Brkan, Katarina. "Karakterizacija i kultivacija knock-out sojeva bakterije Escherichia coli." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:750958

Brkan, K. (2017). 'Karakterizacija i kultivacija knock-out sojeva bakterije Escherichia coli', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 22 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:750958

Brkan K. Karakterizacija i kultivacija knock-out sojeva bakterije Escherichia coli [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2025 January 22] Available at: https://urn.nsk.hr/urn:nbn:hr:159:750958

K. Brkan, "Karakterizacija i kultivacija knock-out sojeva bakterije Escherichia coli", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:750958

Metadata
TitleKarakterizacija i kultivacija knock-out sojeva bakterije Escherichia coli
Title (english)Characterization and cultivation of secreting knock-out strains of Escherichia coli
AuthorKatarina Brkan
MentorBožidar Šantek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2017-12-08, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Escherichia coli is one of the most commonly used microorganisms for production of recombinant proteins due to its numerous advantages. Many of the recombinant proteins E. coli produces are intracellular. To avoid cell disruption, ease protein yielding and purification, there have been attempts in improving extracellular protein production. One of the approaches, applied and investigated in this thesis, is using so-called leaky mutants. Due to mutations in genes encoding for proteins of the cell membrane, these mutants show an increased protein release from periplasm to nutrient medium after protein has been translocated by using natural bacterial translocation systems. Four E. coli knock-out strains have been cultivated first in flasks and then in bioreactors. Strains have been characterized regarding to their growth, protein production and secretion ability, using phytase as reporter protein.
Abstract (croatian)
Escherichia coli je jedan od najčešće korištenih mikroorganizama za proizvodnju rekombinantnih proteina od kojih su mnogi intracelularni. Kako bi se izbjeglo mehaničko razbijanje stanica, olakšala izolacija i pročišćavanje proteina pokušava se unaprijediti proces ekstracelularne proizvodnje proteina. Jedan od pristupa, primijenjen i istražen u ovom radu je korišenje takozvanih leaky mutanata. Zbog mutacija u genima koji kodiraju za proteine stanične stijenke, ovi mutanti bi trebali pokazivati povećanu sposobnost izlučivanja proteina iz periplazme u hranjivu podlogu nakon translokacije proteina prirodno prisutnim bakterijskim translokacijskim mehanizmima. Četiri knock-out soja bakterije E. coli su kultivirani najprije u tikvicama a zatim i u bioreaktorima. Kultivirani sojevi su karakterizirani obzirom na rast, koncentraciju proizvedenog proteina i sposobnost sekrecije koristeći fitazu kao reporter protein.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:159:750958
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-11-21 14:16:11