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master's thesis
Permeabilizacija stanica bakterije Leuconostoc mesenteroides i njihova primjena u proizvodnji alfa-glukozilglicerola
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:382166
Vučenović, Ivan
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Vučenović, I. (2018). Permeabilizacija stanica bakterije Leuconostoc mesenteroides i njihova primjena u proizvodnji alfa-glukozilglicerola (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:382166

Vučenović, Ivan. "Permeabilizacija stanica bakterije Leuconostoc mesenteroides i njihova primjena u proizvodnji alfa-glukozilglicerola." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:382166

Vučenović, Ivan. "Permeabilizacija stanica bakterije Leuconostoc mesenteroides i njihova primjena u proizvodnji alfa-glukozilglicerola." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:382166

Vučenović, I. (2018). 'Permeabilizacija stanica bakterije Leuconostoc mesenteroides i njihova primjena u proizvodnji alfa-glukozilglicerola', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 31 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:382166

Vučenović I. Permeabilizacija stanica bakterije Leuconostoc mesenteroides i njihova primjena u proizvodnji alfa-glukozilglicerola [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 December 31] Available at: https://urn.nsk.hr/urn:nbn:hr:159:382166

I. Vučenović, "Permeabilizacija stanica bakterije Leuconostoc mesenteroides i njihova primjena u proizvodnji alfa-glukozilglicerola", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:382166

Metadata
TitlePermeabilizacija stanica bakterije Leuconostoc mesenteroides i njihova primjena u proizvodnji alfa-glukozilglicerola
Title (english)Permeabilization of cells of bacterium Leuconostoc mesenteroides and their application in production of alfa-glucosylglycerol
AuthorIvan Vučenović
MentorAnita Slavica (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2018-07-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Gram-pozitivna bakterija mliječne kiseline Leuconostoc mesenteroides znatno bolje raste u kemijski djelomično definiranoj podlozi pri aerobnim ( = 0,24 h-1) nego mikroaerofilnim uvjetima ( = 0,02 h-1). Pri aerobnim uvjetima ova bakterija doseže polovinu eksponencijalne faze rasta kroz 5,0 h, kada je koncentracija i aktivnost saharoza fosforilaze, citoplazmatskog enzima koji katalizira transglukozilaciju, maksimalna. U reakcijskoj smjesi u kojoj je istraživana transglukozilacijska aktivnost saharoza fosforilaze, saharoza je donor glukozila, dok je glicerol akceptor glukozila. Međutim, saharoza i glicerol su supstrati za centralni metabolizam u stanicama ove heterofermentativne bakterije mliječne kiseline s pomoću koje je proizvedena samo mliječna kiselina, dok ostali krajnji proizvodi razgradnje ovih supstrata nisu identificirani HPLC metodom. Primjena ultrazvuka i površinski aktivne tvari Tween-80 pokazale su se kao dobre metode, kojima je moguće permeabilizirati bakterijske stanice ali i utjecati na aktivnost ove bakterije. Nakon provedenih postupaka permeabilizacije stanice se dalje eksponencijalno umnažaju i zadržavaju svoju metaboličku aktivnost. U reakcijskoj smjesi s pomoću permeabiliziranih stanica bakterije L. mesenteroides formira se α-glukozilglicerol, koji se eluira između saharoze i glukoze tijekom HPLC analize uzoraka. Nastavak ovog istraživanja uključuje izolaciju proizvedenog proizvoda i njegovu identifikaciju i kvantifikaciju npr. nuklearnom magnetnom rezonancijom.
Abstract (english)
Gram-positive lactic acid bacterium Leuconostoc mesenteroides grew significantly better in chemically semi-defined medium under aerobic ( = 0.24 h-1) than under microaerophilic conditions ( = 0.02 h-1). Under aerobic conditions this bacterium reached half of the exponential growth phase over 5.0 h when concentration and activity of sucrose phosphorylase, cytoplazmatic enzyme that catalyzes transglucosylation reaction, should be maximal. In reaction mixture for the transglucosylation reaction catalyzed by the sucrose phosporylase, sucrose serves as glucosyl donor while glycerol is glucosyl acceptor. However, sucrose and glycerol are also substrates in central metabolism of this heterofermentative lactic acid bacterium that produced only lactic acid, while other end-products of the two substrates degradation were not identified by HPLC method. Application of ultrasound and surfactant Tween-80 are methods of choice and both methods can be used for cell permeabilization but also in directing metabolic activity of the bacterium. After permeabilization the cells continued to grew exponentially and they retained their metabolic activity. In reaction mixture formation of α- glucosylglycerol was catalyzed by the permeabilized cells of the bacterium L. mesenteroides and formed product eluted between sucrose and glucose during HPLC analysis of withdrawn samples. Next steps in this investigation should include isolation of produced α-glucosylglycerol and its unambiguous identification and quantification by e.g. nuclear magnetic resonance.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:382166
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2019-11-26 13:47:49