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master's thesis
Strukturna karakterizacija egzopolisaharida proizvedenih pomoću Lactobacillus fermentum D12
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:039334
Vidulin, Tina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Vidulin, T. (2018). Strukturna karakterizacija egzopolisaharida proizvedenih pomoću Lactobacillus fermentum D12 (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:039334

Vidulin, Tina. "Strukturna karakterizacija egzopolisaharida proizvedenih pomoću Lactobacillus fermentum D12." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:039334

Vidulin, Tina. "Strukturna karakterizacija egzopolisaharida proizvedenih pomoću Lactobacillus fermentum D12." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:039334

Vidulin, T. (2018). 'Strukturna karakterizacija egzopolisaharida proizvedenih pomoću Lactobacillus fermentum D12', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:039334

Vidulin T. Strukturna karakterizacija egzopolisaharida proizvedenih pomoću Lactobacillus fermentum D12 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:159:039334

T. Vidulin, "Strukturna karakterizacija egzopolisaharida proizvedenih pomoću Lactobacillus fermentum D12", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:039334

Metadata
TitleStrukturna karakterizacija egzopolisaharida proizvedenih pomoću Lactobacillus fermentum D12
Title (english)Structural characterization of exopolysaccharides produced BY Lactobacillus fermentum D12
AuthorTina Vidulin
MentorBlaženka Kos (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2018-07-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Egzopolisaharide (EPOL-e), biopolimere široko rasprostranjene u prirodi, bakterije izlučuju u ekstracelularni matriks. Klasificiraju se u dvije glavne skupine: homoegzopolisaharidi (homo-EPOL-i), sastavljeni od jedne vrste monosaharida i heteroegzopolisaharidi (hetero-EPOL-i), sastavljeni od različitih vrsta monosaharida. Cilj ovog rada bio je sinteza EPOL-a pomoću bakterije mliječne kiseline Lactobacillus fermentum D12, ispitivanje utjecaja sastava podloge na sintezu EPOL-a te njihova strukturna analiza. Smjesa izoliranih egzopolisaharida uspješno je razdvojena u tri frakcije kromatografijom visoke djelotvornosti isključenjem na osnovi veličine čestica, a instrumentalne analize sastava i analize glikozidnih veza provedene su na samoj smjesi kao i na svim dobivenim frakcijama. Rezultati analiza pokazali su kako uzorak Lactobac 1 uglavnom sadrži α-glukan koji se sastoji od 1,4- vezane α-D-glukoze, gdje je 20% šećera acetilirano na C-3 položaju. Analiza sastava uzoraka Lactobac 2 i Lactobac 3 pokazala je da se polisaharidi prisutni u uzorcima sastoje od manoze, glukoze i galaktoze u različitim omjerima, dok je analiza glikozidnih veza ukazala na prisutnost nekoliko točaka grananja u strukturi polisaharida.
Abstract (english)
Exopolysaccharides (EPOLs), biopolymers widespread in nature, are extracted by bacteria in the extracellular matrix. They are classified into two main groups: homoexopolysaccharides (homo-EPOL), consisted of one type of monosaccharides, and heteroexopolysaccharides (hetero-EPOL) consisted of different types of monosaccharides. The main goal of this thesis was to synthesize EPOLs with lactic acid bacteria Lactobacillus fermentum D12, to investigate the effect of media composition on EPOLs synthesis and structural characterization of produced EPOLs. Mixture of isolated EPOLs was successfully separated in three fractions by high performance size exclusion chromatography and composition analysis together with linkage analysis was performed both on mixture and on obtained fractions. Results of the analysis showed that sample Lactobac 1 mainly contains an α-glucan consisted of 4-linked α-D-glucose, where 20 % of sugars bear an acetyl substituent on the C-3 position. Furthermore, composition analysis of samples Lactobac 2 and Lactobac 3 showed that present polysaccharides are consisted of mannose, glucose, galactose in different ratios, while glycoside linkage analysis indicates the presence of few branching points in EPOLs.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:039334
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2019-11-27 10:26:38