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master's thesis
Utjecaj prirodnih eutektičkih otapala na lipazom kataliziranu hidrolizu (R, S)-1-feniletil-acetata
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:636580
Horvat, Magdalena
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Horvat, M. (2018). Utjecaj prirodnih eutektičkih otapala na lipazom kataliziranu hidrolizu (R, S)-1-feniletil-acetata (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:636580

Horvat, Magdalena. "Utjecaj prirodnih eutektičkih otapala na lipazom kataliziranu hidrolizu (R, S)-1-feniletil-acetata." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:636580

Horvat, Magdalena. "Utjecaj prirodnih eutektičkih otapala na lipazom kataliziranu hidrolizu (R, S)-1-feniletil-acetata." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:636580

Horvat, M. (2018). 'Utjecaj prirodnih eutektičkih otapala na lipazom kataliziranu hidrolizu (R, S)-1-feniletil-acetata', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 18 February 2025, https://urn.nsk.hr/urn:nbn:hr:159:636580

Horvat M. Utjecaj prirodnih eutektičkih otapala na lipazom kataliziranu hidrolizu (R, S)-1-feniletil-acetata [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2025 February 18] Available at: https://urn.nsk.hr/urn:nbn:hr:159:636580

M. Horvat, "Utjecaj prirodnih eutektičkih otapala na lipazom kataliziranu hidrolizu (R, S)-1-feniletil-acetata", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:636580

Metadata
TitleUtjecaj prirodnih eutektičkih otapala na lipazom kataliziranu hidrolizu (R, S)-1-feniletil-acetata
Title (english)The effect of deep eutectic solvents on lipase catalyzed hydrolysis of (R, S)-1 phenylethyl-acetate
AuthorMagdalena Horvat
MentorMarina Cvjetko Bubalo (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2018-10-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Prirodna eutektička otapala mogu se primijeniti u biotransformacijama za proizvodnju biološki aktivnih kiralnih spojeva ili kiralnih sintona visoke enantiomerne čistoće. Tako se u ovom radu ispitivala mogućnost primjene dva pristupa zelene tehnologije u sintezi (R)-1-feniletanola: (i) primjena lipaze B kao biokatalizatora u hidrolizi (R, S)-1-feniletil-acetata (ii) primjena prirodnih eutektičkih otapalima na bazi kolin-klorida kao medija za provođenje hidrolize (R, S)-1-feniletil-acetata. Praćenjem parametara (η, ee), zaključuje se da je najpovoljnije otapalo za lipazom kataliziranu enantioselektivnu hidrolizu prirodno eutektičko otapalo kolin-klorid:glukoza s 50 % (v/v) vode (ChGlc50). Nadalje, optimirana je reakcija enantioselektivne hidrolize (R, S)-1-feniletilacetata u prirodnom eutektičkom ChGlc50. Optimirana je temperatura reakcije te omjer enzima i supstrata. Istražen je i mehanizam reakcije te je određen utjecaj alternativnih izvora energije (ultrazvuka i mikrovalova) na tijek reakcije.
Abstract (english)
Natural eutectic solvents can be applied in biotransformations for the production of biologically active chiral compounds or chiral syntones of high enantiomeric purity. In this graduate thesis it is explored the possibility of applying two approaches to green technology in synthesis of (R) -1-phenylethanol: (i) the use of lipase B as a hydrolysis (R, S) -1-phenylethyl acetate hydrolysis (ii) applying aprioric eutectic solvents to choline chloride base as a medium for the hydrolysis (R, S) -1-phenylethyl acetate. By monitoring parameters (η, ee) it is concluded that the most preferred solvent for lipase-catalyzed enantioselective hydrolysis is a natural eutectic solvent choline chloride: 50 % (v / v) water glucose (ChGlc50). Furthermore, the enantioselective hydrolysis reaction (R, S)-1-phenylethyl acetate in the natural eutectic ChGlc50 was optimized. The reaction temperature and the enzyme and substrate ratio are optimized. The mechanism of reaction was also studied and the influence of alternative energy sources (ultrasound and microwaves) on the reaction course was determined.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:636580
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
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Created on2019-11-27 11:27:13