prikaz prve stranice dokumenta Izolacija pigmenata iz alge Chlorella vulgaris primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku
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master's thesis
Izolacija pigmenata iz alge Chlorella vulgaris primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:195623
Simonović, Niki
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Food Engineering
Laboratory for Technology of Fruits and Vegetables Preservation and Processing

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Simonović, N. (2018). Izolacija pigmenata iz alge Chlorella vulgaris primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:195623

Simonović, Niki. "Izolacija pigmenata iz alge Chlorella vulgaris primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:195623

Simonović, Niki. "Izolacija pigmenata iz alge Chlorella vulgaris primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:195623

Simonović, N. (2018). 'Izolacija pigmenata iz alge Chlorella vulgaris primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 30 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:195623

Simonović N. Izolacija pigmenata iz alge Chlorella vulgaris primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 December 30] Available at: https://urn.nsk.hr/urn:nbn:hr:159:195623

N. Simonović, "Izolacija pigmenata iz alge Chlorella vulgaris primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:195623

Metadata
TitleIzolacija pigmenata iz alge Chlorella vulgaris primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku
Title (english)Isolation of pigments from algae Chlorella vulgaris using accelerated solvent extraction
AuthorNiki Simonović
MentorVerica Dragović Uzelac (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Food Engineering)
(Laboratory for Technology of Fruits and Vegetables Preservation and Processing)
Zagreb
Defense date and country2018-09-24, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Abstract
Cilj ovog istraživanja bio je utvrditi utjecaj broja ciklusa (1, 2), temperature (100, 130, 180 °C) i vremena (5, 10, 15 min) na prinos klorofila i karotenoida ekstrahiranih iz zelene alge Chlorella vulgaris primjenom ubrzane ekstrakcije otapalima uz povišeni tlak (PLE), uz etanol kao ekstrakcijsko otapalo. Nadalje, cilj je bio optimirati parametre PLE pri kojima će se ostvariti najveći prinosi klorofila i karotenoida. Optimalni uvjeti ekstrakcije klorofila a postignuti su pri temperaturi 160 °C, vremenu ekstrakcije 12 minuta kroz 2 ekstrakcijska ciklusa, a za klorofil b pri 130 °C, 9 minuta i 1 ekstrakcijski ciklus. Optimalni uvjeti pri kojima se dobivaju veći prinosi karotenoida su temperatura 140 °C, vrijeme ekstrakcije 5 minuta i 2 ekstrakcijska ciklusa. Masena koncentracija klorofila a pri optimalnim uvjetima iznosi 14,11 mg g-1 s.t., klorofila b 5,69 mg g-1 s.t., a karotenoida 2,15 mg g-1 s.t. Na temelju statističke analize MANOVA utvrđeno je da svi istraživani parametri imaju značajan utjecaj na prinose istraživanih pigmenata. Pomoću regresijskih modela određene su predviđene vrijednosti pigmenata u ekstraktu koje su eksperimentalno potvrđene.
Abstract (english)
The aim of this study was to determine the influence of number of cycles (1, 2), temperature (100, 130, 180 ° C) and time (5, 10, 15 min) on the yield of chlorophyll and carotenoid extracted from green algae Chlorella vulgaris by accelerated extraction with elevated pressure (PLE), with ethanol as an extraction solvent. Furthermore, the aim was to optimize PLE parameters, with the highest yields of chlorophyll and carotenoid. Optimal conditions of chlorophyll extraction were achieved at 160 ° C, 12 min extraction time for 2 extraction cycles and for chlorophyll b at 130 ° C for 9 minutes and 1 extraction cycle. The optimal conditions for obtaining higher carotenoid yields are 140 ° C, 5 min extraction time and 2 extraction cycles. The mass concentration of chlorophyll at optimal conditions was 14.11 mg g-1 d.m., chlorophyll b 5.69 mg g-1 d.m., and carotenoid 2.15 mg g-1 d.m. Based on the MANOVA statistical analysis, all the parameters investigated have a significant effect on the yields of the investigated pigments. Regression models provided predicted values of pigments in the extract that were experimentally verified.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:195623
Study programmeTitle: Food Safety Management
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2019-12-02 14:27:56