prikaz prve stranice dokumenta Identifikacija bakterija mliječne kiseline izoliranih iz probiotičkih proizvoda
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undergraduate thesis
Identifikacija bakterija mliječne kiseline izoliranih iz probiotičkih proizvoda
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. urn:nbn:hr:159:540840
Vešligaj, Martina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Vešligaj, M. (2014). Identifikacija bakterija mliječne kiseline izoliranih iz probiotičkih proizvoda (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:540840

Vešligaj, Martina. "Identifikacija bakterija mliječne kiseline izoliranih iz probiotičkih proizvoda." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:540840

Vešligaj, Martina. "Identifikacija bakterija mliječne kiseline izoliranih iz probiotičkih proizvoda." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:540840

Vešligaj, M. (2014). 'Identifikacija bakterija mliječne kiseline izoliranih iz probiotičkih proizvoda', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 18 February 2025, https://urn.nsk.hr/urn:nbn:hr:159:540840

Vešligaj M. Identifikacija bakterija mliječne kiseline izoliranih iz probiotičkih proizvoda [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2014 [cited 2025 February 18] Available at: https://urn.nsk.hr/urn:nbn:hr:159:540840

M. Vešligaj, "Identifikacija bakterija mliječne kiseline izoliranih iz probiotičkih proizvoda", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:159:540840

Metadata
TitleIdentifikacija bakterija mliječne kiseline izoliranih iz probiotičkih proizvoda
Title (english)Identification of lactic acid bacteria isolated from probiotic products
AuthorMartina Vešligaj
MentorJagoda Šušković (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2014-06-17, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Jedanaest bakterijskih sojeva, izoliranih iz komercijalno dostupnih probiotičkih pripravaka na hrvatskom tržištu je odabrano za reidentifikaciju. Klasičnim mikrobiološkim metodama su sojevi okarakterizirani kao gram-pozitivni, nesporogeni i katalaza-negativni. RAPD metoda je provedena s početnicama za bakterije mliječne kiseline kako bi potvrdila raznolikost izoliranih sojeva. Identifikacija na razini roda je provedena PCR reakcijama s početnicama za rodove Lactobacillus i Bifidobacterium. Sojevi 1, 2a, 4, 5, 6, 8/1, 8/2 i 9 su pokazali pozitivan signal za rod Lactobacillus, a sojevi 3 i 8/2 pozitivan signal za rod Bifidobacetirum. Fenotipska identifikacija je provedenim API 50 CHL testom. Sojevi 1 i 2a su identificirani kao Lactobacillus rhamnosus, a sojevi 4, 5, 6 i 9 kao Lactobacillus fermentum što odgovara deklaracijama proizvoda. Za soj 8/2, koji je genetičkim metodama povrđen kao mješoviti soj, API sustav je potvrdio neprihvatljiv profil. Tijekom uzgoja izoliranih bakterijskih sojeva u tekućim podlogama praćeni su parametri proizvodnje mliječne kiseline i promjene pH vrijednosti.
Abstract (english)
Eleven strains were isolated from commercially available probiotic preparations on the Croatian market and selected for reidentification. The classical microbiological methods characterized strains as Gram-positive, nonsporogenic and catalase-negative. RAPD method with primers for lactic acid bacteria was used in order to confirm diversity amonf isolated strains. Identification at the genus level was confirmed by PCR reactions with primers for the Lactobacillus and Bifidobacterium genera. Strains 1, 2a, 4, 5, 6, 8/1, 8/2 and 9 showed the positive signal for the Lactobacillus genus and strains 3 and 8/2 showed positive signals for the Bifidobacetirum genus. Phenotypic identification was performed with API 50 CHL test. Strains 1 and 2a were identified as Lactobacillus rhamnosus, and strains 4, 5, 6 and 9 as Lactobacillus fermentum which corresponds to the products label. Strain 8/2 was defined as mix culture with genetic methods and API system confirmed his unacceptable profile. Parameters and production of lactic acid and the pH value were also monitored during the cultivation of isolated bacterial strains in liquid media.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:540840
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-26 10:48:30