prikaz prve stranice dokumenta Uzgoj kvasca Scheffersomyces stipitis na laboratorijskoj tresilici i u laboratorijskom bioreaktoru s ciljem proizvodnje (S)-adenozil-L- metionina
  Download
PDF 1.85 MB
master's thesis
Uzgoj kvasca Scheffersomyces stipitis na laboratorijskoj tresilici i u laboratorijskom bioreaktoru s ciljem proizvodnje (S)-adenozil-L- metionina
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. urn:nbn:hr:159:034586
Kralj, Elizabeta
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Food Engineering
Laboratory for Fermentation and Yeast Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Kralj, E. (2016). Uzgoj kvasca Scheffersomyces stipitis na laboratorijskoj tresilici i u laboratorijskom bioreaktoru s ciljem proizvodnje (S)-adenozil-L- metionina (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:034586

Kralj, Elizabeta. "Uzgoj kvasca Scheffersomyces stipitis na laboratorijskoj tresilici i u laboratorijskom bioreaktoru s ciljem proizvodnje (S)-adenozil-L- metionina." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:034586

Kralj, Elizabeta. "Uzgoj kvasca Scheffersomyces stipitis na laboratorijskoj tresilici i u laboratorijskom bioreaktoru s ciljem proizvodnje (S)-adenozil-L- metionina." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:034586

Kralj, E. (2016). 'Uzgoj kvasca Scheffersomyces stipitis na laboratorijskoj tresilici i u laboratorijskom bioreaktoru s ciljem proizvodnje (S)-adenozil-L- metionina', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 01 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:034586

Kralj E. Uzgoj kvasca Scheffersomyces stipitis na laboratorijskoj tresilici i u laboratorijskom bioreaktoru s ciljem proizvodnje (S)-adenozil-L- metionina [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2016 [cited 2024 June 01] Available at: https://urn.nsk.hr/urn:nbn:hr:159:034586

E. Kralj, "Uzgoj kvasca Scheffersomyces stipitis na laboratorijskoj tresilici i u laboratorijskom bioreaktoru s ciljem proizvodnje (S)-adenozil-L- metionina", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:159:034586

Metadata
TitleUzgoj kvasca Scheffersomyces stipitis na laboratorijskoj tresilici i u laboratorijskom bioreaktoru s ciljem proizvodnje (S)-adenozil-L- metionina
Title (english)Cultivation of yeast Scheffersomyces stipitis in laboratory shaker and in laboratory bioreactor with purpose of (S)-adenosyl-L-methionine production
AuthorElizabeta Kralj
MentorDamir Stanzer (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Food Engineering)
(Laboratory for Fermentation and Yeast Technology)
Zagreb
Defense date and country2016-07-13, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
(S)-adenozil-L-metionin (SAMe, AdoMet) je glavni donor metilne skupine u reakcijama kataliziranim enzimima metiltransferazama te sudjeluje u metilaciji DNA, RNA, sterola i fosfolipida. Kod ljudi se poremećaji u metabolizmu SAMe povezuju s različitim oboljenjima pa ima značajnu ulogu kao farmaceutik i nutraceutik u liječenju depresije, osteoartritisa, bolesti jetre i Alzheimerove bolesti. Najčešće se proizvodi mikrobiološkom sintezom pomoću kvasaca. U ovom radu uzgajana je biomasa soja M12 kvasca Scheffersomyces stipitis na laboratorijskoj tresilici i u laboratorijskom bioreaktoru. S ciljem ispitivanja utjecaja dodatka protektivnih spojeva (glicerola, mioinozitola i kolina) na rast biomase i udio SAMe uzgoji su provedeni u tzv. O-podlozi bez i uz dodatak protektivnih spojeva. Dodatak glicerola u O-podlogu u uvjetima povećane aeracije pozitivno je djelovao na rast soja M12 kao i na udio SAMe u kvascu uzgojenom na laboratorijskoj tresilici. Uzgojem soja M12 kvasca S. stipitis u laboratorijskom bioreaktoru u modificiranoj O-podlozi s glicerolom kao osnovnim izvorom ugljika postignut je dobar rast soja M12 kvasca S. stipitis, a uz pulsni dodatak D,L-metionina na početku stacionarne faze ostvareno je povećanje udjela SAMe u kvaščevoj biomasi.
Abstract (english)
(S)-adenosyl-L-methionine (SAMe, AdoMet) is a primary donor of methyl group in reactions catalyzed by methyltransferases and is directly involved in methylation of DNA, RNA, sterols and phospholipids. Disorders in SAMe metabolism are associated with various diseases in humans, so it has an important role as a pharmaceutical and a nutraceutical in treatments of conditions such as depression, osteoarthritis, liver diseases and Alzheimer's disease. SAMe is most commonly produced by microbial synthesis using yeasts. In this study a biomass of yeast strain Scheffersomyces stipitis M12 was grown in laboratory shaker and in laboratory bioreactor. Cultivations were carried out in O-medium without and with addition of protective compounds, so the impact of this compounds (glycerol, myo-inositol and choline) on the growth of biomass and on the SAMe content could have been tested. Addition of glycerol in O-medium in conditions of increased aeration positively affected the growth of M12 strain as well as SAMe content in the laboratory shaker. During the cultivation of M12 strain in a laboratory bioreactor in the modified O-medium with glycerol as a primary carbon source a satisfactory amount of M12 biomass was produced, while pulsed addition of D, L-methionine at the start of the stationary phase increased the SAMe content in biomass.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:034586
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-26 11:11:32