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master's thesis
Fiziologija heterofermentativnog soja Leuconostoc mesenteroides LMG 7954 pri različitim uvjetima uzgoja šaržnim postupkom u laboratorijskom mjerilu
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. urn:nbn:hr:159:615416
Gruičić, Ana-Marija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Gruičić, A. M. (2019). Fiziologija heterofermentativnog soja Leuconostoc mesenteroides LMG 7954 pri različitim uvjetima uzgoja šaržnim postupkom u laboratorijskom mjerilu (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:615416

Gruičić, Ana Marija. "Fiziologija heterofermentativnog soja Leuconostoc mesenteroides LMG 7954 pri različitim uvjetima uzgoja šaržnim postupkom u laboratorijskom mjerilu." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:615416

Gruičić, Ana Marija. "Fiziologija heterofermentativnog soja Leuconostoc mesenteroides LMG 7954 pri različitim uvjetima uzgoja šaržnim postupkom u laboratorijskom mjerilu." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:615416

Gruičić, A. M. (2019). 'Fiziologija heterofermentativnog soja Leuconostoc mesenteroides LMG 7954 pri različitim uvjetima uzgoja šaržnim postupkom u laboratorijskom mjerilu', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 09 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:615416

Gruičić AM. Fiziologija heterofermentativnog soja Leuconostoc mesenteroides LMG 7954 pri različitim uvjetima uzgoja šaržnim postupkom u laboratorijskom mjerilu [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2019 [cited 2024 December 09] Available at: https://urn.nsk.hr/urn:nbn:hr:159:615416

A. M. Gruičić, "Fiziologija heterofermentativnog soja Leuconostoc mesenteroides LMG 7954 pri različitim uvjetima uzgoja šaržnim postupkom u laboratorijskom mjerilu", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:159:615416

Metadata
TitleFiziologija heterofermentativnog soja Leuconostoc mesenteroides LMG 7954 pri različitim uvjetima uzgoja šaržnim postupkom u laboratorijskom mjerilu
Title (english)Physiology of heterofermentative strain Leuconostoc mesenteroides LMG 7954 under different conditions of batch cultivation in the laboratory scale
AuthorAna-Marija Gruičić
MentorAnita Slavica (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2019-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U istraţivanjima u ovom radu utvrĎen je utjecaj koncentracije otopljenog kisika na rast soja Leuconostoc mesenteroides LMG 7954 u kemijski djelomično definiranoj podlozi (Vandamme i sur., 1987.) pri 28±1,0°C i opadajućoj pH vrijednosti suspenzije tijekom uzgoja šarţnim postupkom u laboratorijskom mjerilu. Razvijena je i optimirana ionsko-izmjenjivačka HPLC metoda, kojom je popraćena aktivnost ovog soja u različitim uvjetima uzgoja šarţnim postupkom. L. mesenteroides LMG 7954 uspješno raste u hranjivoj MRS podlozi, kemijski djelomično definiranoj hranjivoj podlozi kao i u smjesi visokog osmotskog tlaka saharoza:glicerol. Ovaj soj moţe transportirati i metabolizirati glukozu, saharozu (glukozu i fruktozu) i glicerol. Glavni proizvodi metabolizma ovih ugljikohidrata i alkohola su bakterijska biomasa i laktat te znatno manje koncentracije acetata i etanola. U smjesi saharoza:glicerol ova Gram-pozitivna bakterija raste slijedeći eksponencijalni trend, a zatim linearni trend. Ova vaţna fiziološka karakteristika kvalificira ovaj soj za industrijsku primjenu u podlogama s visokim početnim koncentracijama supstrata. Primjenom ultrazvuka i dodatkom Tween-a-80 permeabilizirane su stanice ovog soja i to tijekom njihova rasta u smjesi saharoza:glicerol. Obje metode utječu na metabolizam supstrata u stanicama ove (navodno) mikroaerofilne heterofermentativne bakterije mliječne kiseline, kako je to utvrĎeno HPLC metodom. Nadalje, identificiran je dodatni pik u kromatogramima, koji ukazuje na mogućnost proizvodnje -glukozilglicerola s pomoću ovog soja.
Abstract (english)
In investigation described in this work effect of dissolved oxygen on the growth of strain Leuconostoc mesenteroides LMG 7954 in chemically semi-defined broth (Vandamme et al., 1987) was determined at 281.0°C and decreasing pH value of suspension during batch cultivation in the laboratory scale. Ion-exchange HPLC method was developed and optimized in order to follow activity of the strain under different conditions of the batch cultivation. L. mesenteroides LMG 7954 grew in MRS broth, the chemically semi-defined broth and sucrose:glycerol mixture with high osmotic pressure. The strain is able to transport and metabolize glucose, sucrose (glucose and fructose) and glycerol. The main end-products of metabolism of those carbohydrates and alcohol are bacterial biomass and lactate, while significantly lower concentration of acetate and ethanol were detected. In the sucrose:glycerol mixture this Gram-positive bacterium grew first exponentially and then followed linear trend. This physiological characteristic can be very useful in industrial application of the strain, especially in the cultivations with high initial concentration of substrate(s). Cells of this strain were permeabilized when growing in the sucrose:glycerol mixture by using ultrasound treatment and addition of Tween-80. Metabolism of the substrates in the cells of these (apparently) microaerophilic heterofermenative lactic acid bacterium was affected by both methods, as determined by HPLC method. Furthermore, additional peak was identified within HPLC chromatograms which indicates possibility for production of -glucosyglycerol by the strain.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:615416
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2019-12-16 13:22:00