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master's thesis
Enzimska hidroliza predobrađenih kukuruznih oklasaka i proizvodnja etanola pomoću plijesni Mucor indicus
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. urn:nbn:hr:159:846655
Fičko, Valentina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Fičko, V. (2019). Enzimska hidroliza predobrađenih kukuruznih oklasaka i proizvodnja etanola pomoću plijesni Mucor indicus (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:846655

Fičko, Valentina. "Enzimska hidroliza predobrađenih kukuruznih oklasaka i proizvodnja etanola pomoću plijesni Mucor indicus." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:846655

Fičko, Valentina. "Enzimska hidroliza predobrađenih kukuruznih oklasaka i proizvodnja etanola pomoću plijesni Mucor indicus." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:846655

Fičko, V. (2019). 'Enzimska hidroliza predobrađenih kukuruznih oklasaka i proizvodnja etanola pomoću plijesni Mucor indicus', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 17 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:846655

Fičko V. Enzimska hidroliza predobrađenih kukuruznih oklasaka i proizvodnja etanola pomoću plijesni Mucor indicus [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2019 [cited 2024 June 17] Available at: https://urn.nsk.hr/urn:nbn:hr:159:846655

V. Fičko, "Enzimska hidroliza predobrađenih kukuruznih oklasaka i proizvodnja etanola pomoću plijesni Mucor indicus", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:159:846655

Metadata
TitleEnzimska hidroliza predobrađenih kukuruznih oklasaka i proizvodnja etanola pomoću plijesni Mucor indicus
Title (english)Enzymatic hydrolysis of pretreated corn cobs and ethanol production by Mucor indicus
AuthorValentina Fičko
MentorMario Novak (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2019-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Lignocelulozne sirovine poput kukuruznih oklasaka najčešće ostaju neiskorištene, a jeftina su sirovina za proizvodnju biokemikalija kao što je bioetanol. Glavni problem upotrebe takvih sirovina u različitim procesima je potreba provođenja postupaka predobrade i hidrolize koji u konačnici poskupljuju ukupni proces proizvodnje. U ovom radu provedena je enzimska hidroliza (pri 50 °C; pH 4,8) kiselinski predobrađenih kukuruznih oklasaka (s 10-40 g L-1 sirovine) pomoću enzima Viscozyme L i Cellulase, enzyme blend (1-20 % vol vol-1). Stupanj hidrolize od 85 % postignut je kombinacijom 2 % vol vol-1 Viscozyme L i 5 % Cellulase, enzyme blend. Nakon optimizacije enzimske hidrolize, dobiveni hidrolizat korišten je za uzgoj plijesni Mucor indicus te proizvodnju etanola u HRCB-u i barbotirajućem bioreaktoru. Uspješnija proizvodnja bioetanola postignuta je u barbotirajućem bioreaktoru uz ostvarene pokazatelje uspješnosti procesa: YP/S = 0,46 g g-1; Pr = 0,40 g L-1 h-1; E = 90,20 %.
Abstract (english)
Lignocellulosic raw materials like corn cobs are mostly left unused, but they are a cheap raw material for the production of biochemicals, e.g. bioethanol. The main problem with the utilization of such raw materials in different bioprocesses lies in the necessary pretretments and hydrolysis that ultimately increase the cost of the overall production process. In this work, enzymatic hydrolysis (at 50 °C; pH 4.8) of dilute acid pretreated corn cobs (with 10-40 g L-1) was performed using Viscozyme L and Cellulase, enzyme blend (1-20 % vol vol-1). A hydrolysis rate of 85 % was obtained with the combination of 2 % vol vol-1 Viscozyme L and 5 % vol vol-1 Cellulase, enzyme blend. After the optimization of enzymatic hydrolysis, the resulting hydrolysate was used for the cultivation of Mucor indicus and the production of ethanol in HRCB and bubbling bioreactor. Comparing the two production processes, bioethanol production in the bubbling bioreactor was more successful, with the following obtained bioprocess efficiency parameters: YP/S = 0.46 g g-1; Pr = 0.40 g L-1 h-1; E = 90.20 %.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:846655
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-01-29 14:42:11