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undergraduate thesis
Bakteriocinska aktivnost autohtonih Lactobacillus plantarum sojeva izoliranih iz različitih fermentiranih proizvoda
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:489064
Gagić, Antonio
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Gagić, A. (2020). Bakteriocinska aktivnost autohtonih Lactobacillus plantarum sojeva izoliranih iz različitih fermentiranih proizvoda (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:489064

Gagić, Antonio. "Bakteriocinska aktivnost autohtonih Lactobacillus plantarum sojeva izoliranih iz različitih fermentiranih proizvoda." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:489064

Gagić, Antonio. "Bakteriocinska aktivnost autohtonih Lactobacillus plantarum sojeva izoliranih iz različitih fermentiranih proizvoda." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:489064

Gagić, A. (2020). 'Bakteriocinska aktivnost autohtonih Lactobacillus plantarum sojeva izoliranih iz različitih fermentiranih proizvoda', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:489064

Gagić A. Bakteriocinska aktivnost autohtonih Lactobacillus plantarum sojeva izoliranih iz različitih fermentiranih proizvoda [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:159:489064

A. Gagić, "Bakteriocinska aktivnost autohtonih Lactobacillus plantarum sojeva izoliranih iz različitih fermentiranih proizvoda", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:489064

Metadata
TitleBakteriocinska aktivnost autohtonih Lactobacillus plantarum sojeva izoliranih iz različitih fermentiranih proizvoda
Title (english)Bacteriocin activity of autochthonous Lactobacillus plantarum strains isolated from various fermented products
AuthorAntonio Gagić
MentorJagoda Šušković (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2020-09-01, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Bakteriocini su mali ribosomski sintetizirani peptidi koji inhibiraju rast srodnih mikroorganizama, onemogućavanjem normalnog funkcioniranja staničnih struktura. U ovom radu proučavani su plantaricini, proizvedeni s tri različita soja Lactobacillus plantarum. Antimikrobno djelovanje sojeva ispitivano je na test-mikroorganizmima Listeria monocytogenes ATCC 19111 i Staphylococcus aureus 3048, koji su učestali patogeni. Ispitivanje antibakterijskog djelovanja plantaricina temeljeno je na dvije metode: metodi s rupama u agaru i metodi s dvostrukim slojem agara. Rezultati dobiveni navedenim metodama ukazali su na najveću inhibicijsku sposobnost soja Lb. plantarum M92C. Također, u svrhu ostvarivanja bolje spoznaje aktivnosti i funkcije plantaricina, provedena je analiza gena proučavanih triju Lactobacillus plantarum sojeva pomoću PCR metode. Pritom je PCR metodom dokazana prisutnost gena plnA, plnEF i plnJ koji kodiraju za plantaricine kod svih proučavanih Lb. plantarum sojeva.
Abstract (english)
Bacteriocins are small ribosomally synthesized peptides which inhibit growth of closely related strains of microorganisms, by disrupting normal functioning of important cellular structures. In this research, we examined plantaricins produced by three different strains of Lb. plantarum. Antimicrobial activity was examined on test-microorganisms, Listeria monocytogenes ATCC 19111 and Staphylococcus aureus 3048, which are common pathogens. Assaying for plantaricin activities of three examined Lb. plantarum strains were based on two methods: agar well diffusion method and agar spot test method. Results obtained by these methods demonstrated strongest inhibitory ability of Lb. plantarum M92C. Moreover, to acquire better understanding of plantaricin activity and function, gene analysis was conducted by PCR method for three strains of Lb. plantarum that we used. PCR method demonstrated presence of genes plnA, plnEF and plnJ which code for plantaricines in all Lb. plantarum strains used in analysis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:489064
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-10-29 12:52:13