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master's thesis
Identifikacija i karakterizacija bioaktivnih peptida proizvedenih s odabranim sojevima bakterija mliječne kiseline
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:268124
Brajković, Katarina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Brajković, K. (2020). Identifikacija i karakterizacija bioaktivnih peptida proizvedenih s odabranim sojevima bakterija mliječne kiseline (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:268124

Brajković, Katarina. "Identifikacija i karakterizacija bioaktivnih peptida proizvedenih s odabranim sojevima bakterija mliječne kiseline." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:268124

Brajković, Katarina. "Identifikacija i karakterizacija bioaktivnih peptida proizvedenih s odabranim sojevima bakterija mliječne kiseline." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:268124

Brajković, K. (2020). 'Identifikacija i karakterizacija bioaktivnih peptida proizvedenih s odabranim sojevima bakterija mliječne kiseline', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 06 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:268124

Brajković K. Identifikacija i karakterizacija bioaktivnih peptida proizvedenih s odabranim sojevima bakterija mliječne kiseline [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 June 06] Available at: https://urn.nsk.hr/urn:nbn:hr:159:268124

K. Brajković, "Identifikacija i karakterizacija bioaktivnih peptida proizvedenih s odabranim sojevima bakterija mliječne kiseline", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:268124

Metadata
TitleIdentifikacija i karakterizacija bioaktivnih peptida proizvedenih s odabranim sojevima bakterija mliječne kiseline
Title (english)Identification and characterization of bioactive peptides produced by selected strains of lactic acid bacteria
AuthorKatarina Brajković
MentorBlaženka Kos (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2020-10-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Bioaktivni peptidi podrijetlom iz kazeina i proteina sirutke najbrojnija su skupina bioaktivnih tvari prisutnih u mliječnim proizvodima. To su proteinski fragmenti koji nastaju tijekom probave u humanom organizmu ili tijekom procesa prerade mlijeka metabolizamskom aktivnošću prisutnih bakterija mliječne kiseline. Cilj ovog rada bio je izolacija, pročišćavanje i razdjeljivanje bioaktivnih peptida iz uzoraka svježih sušenih sireva i sirutki kromatografijom ionske izmjene, tekućinskom kromatografijom isključivanja po veličini i spektrometrijom masa na MALDI-TOF/TOF uređaju. Ispitano je antimikrobno i antioksidativno djelovanje izoliranih bioaktivnih peptida. Također, provedena je neciljana analiza i identifikacija proteina iz uzoraka sira i sirutke u svrhu karakterizacije peptida s bioaktivnim djelovanjem. Rezultati provedenih metoda ne pokazuju antimikrobno i antioksidativno djelovanje izoliranih bioaktivnih peptida. Međutim, neciljanom analizom proteina identificirani su bioaktivni peptidi čije aminokiselinske sekvence odgovaraju bioaktivnim peptidima iz baze podataka, a za koje je ranije utvrđeno i u literaturi opisano: ACE-inhibitorno, imunomodulatorno, antitrombotično, antioksidativno i antimikrobno djelovanje.
Abstract (english)
Bioactive peptides originated from casein and whey proteins are the most numerous group of bioactive substances present in dairy products. They are protein fragments that are synetised in the food digestion process in human body or during milk processing by the metabolic activity of the lactic acid bacteria. The aim of this study was to isolate, purify, and separate bioactive peptides from samples of fresh dried cheeses and whey by ion-exchange chromatography, size-exclusion chromatography, and MALDI-TOF/TOF mass spectrometry. The antimicrobial and antioxidant activities of isolated bioactive peptides were tested. Also, non-targeted analysis and identification of proteins from cheese and whey were performed to characterize peptides with bioactive activity. The results of the implemented methods do not show the antimicrobial and antioxidant activity of isolated bioactive peptides. However, non-targeted protein analysis identified bioactive peptides whose amino acid sequences correspond to bioactive peptides from the database. As previously identified, their activities were described in the literature as ACE-inhibitory, immunomodulatory, antithrombotic, antioxidant, and antimicrobial activity.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:268124
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2020-11-17 12:21:28