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master's thesis
Određivanje optimalne temperature za rast i proizvodnju etanola s pomoću kvasca Kluyveromyces marxianus NBRC 1777
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:135984
Samardžić, Ana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Samardžić, A. (2020). Određivanje optimalne temperature za rast i proizvodnju etanola s pomoću kvasca Kluyveromyces marxianus NBRC 1777 (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:135984

Samardžić, Ana. "Određivanje optimalne temperature za rast i proizvodnju etanola s pomoću kvasca Kluyveromyces marxianus NBRC 1777." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:135984

Samardžić, Ana. "Određivanje optimalne temperature za rast i proizvodnju etanola s pomoću kvasca Kluyveromyces marxianus NBRC 1777." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:135984

Samardžić, A. (2020). 'Određivanje optimalne temperature za rast i proizvodnju etanola s pomoću kvasca Kluyveromyces marxianus NBRC 1777', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 29 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:135984

Samardžić A. Određivanje optimalne temperature za rast i proizvodnju etanola s pomoću kvasca Kluyveromyces marxianus NBRC 1777 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2024 June 29] Available at: https://urn.nsk.hr/urn:nbn:hr:159:135984

A. Samardžić, "Određivanje optimalne temperature za rast i proizvodnju etanola s pomoću kvasca Kluyveromyces marxianus NBRC 1777", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:135984

Metadata
TitleOdređivanje optimalne temperature za rast i proizvodnju etanola s pomoću kvasca Kluyveromyces marxianus NBRC 1777
Title (english)Determination of optimal temperature for growth and ethanol production by yeast Kluyveromyces marxianus NBRC 1777
AuthorAna Samardžić
MentorAntonija Trontel (mentor)
Committee memberMario Novak (predsjednik povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2020-12-18, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Razvoj bioprocesa proizvodnje etanola trebao bi biti usmjeren prema pronalaženju termotolerantnog kvasca koji može koristiti različite ugljikohidrate kao izvore ugljika, posebice one prisutne u lignoceluloznim sirovinama, proizvoditi etanol uz visoke prinose i produktivnost pri temperaturama koje su optimalne za aktivnost enzima potrebnih za hidrolizu lignoceluloznih sirovina (≈ 50 °C). Kvasac Kluyveromyces marxianus je termotolerantni kvasac koji može rasti i pri temperaturi od 45 °C do 52 °C, a etanol može proizvoditi pri temperaturama od 38 °C do 45 °C ovisno o odabranom soju ovog kvasca. U ovom radu su zbog pronalaženja optimalne temperature za rast i proizvodnju etanola s pomoću kvasca Kluyveromyces marxianus NBRC 1777 provedeni uzgoji u YPD (Yeast extract pepton dextrose) podlozi pri temperaturama od 20 °C, 30 °C, 40 °C i 50 °C. Između testiranih temperatura, najveća specifična brzina rasta (μ = 0,42 hˉ¹) te brzine proizvodnje etanola (rEtOH = 0,35 hˉ¹) i glicerola (rglicerol = 0,19 hˉ¹), kao i produktivnost procesa proizvodnje etanola (PrEtOH =0,96 g Lˉ¹ hˉ¹) određene su pri temperaturi od 30 °C. Pri temperaturi od 50 °C ovaj soj kvasca raste vrlo sporo i proizvodi minimalne količine etanola uz vrlo nisku produktivnost procesa proizvodnje etanola (PrEtOH = 0,15 g Lˉ¹ hˉ¹).
Abstract (english)
The development of ethanol production bioprocess should be aimed at finding thermotolerant yeast that can use various carbohydrates as carbon sources, especially those present in lignocellulosic feedstocks, produce ethanol with high yields and productivity at temperatures optimal for the activity of enzymes required for hydrolysis of lignocells (≈ 50 °C). Yeast Kluyveromyces marxianus is a thermotolerant yeast that can grow at temperatures from 45 °C to 52 °C and ethanol can be produced at temperatures from 38 °C to 45 °C depending on the selected strain of this yeast. In this work, in order to find the optimal temperature for the growth and production of ethanol with the help of yeast Kluyveromyces marxianus NBRC 1777, cultivations were performed in YPD (Yeast extract pepton dextrose) medium at temperatures of 20 °C, 30 °C, 40 °C and 50 °C. Between the tested temperatures, the highest specific growth rate (μ = 0.42 hˉ¹) and the production rate of ethanol (rEtOH = 0.35 hˉ¹) and glycerol (rglycerol = 0.19 hˉ¹), as well as the productivity of the production process ethanol (PrEtOH = 0.96 g Lˉ¹ hˉ¹) were determined at 30 °C. At a temperature of 50 °C this yeast strain grows very slowly and produces minimal amounts of ethanol with very low productivity of the ethanol production process (PrEtOH = 0.15 g Lˉ¹ hˉ¹).
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:135984
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2021-01-11 09:37:18