prikaz prve stranice dokumenta Priprema polifenolnih ekstrakata iz otpada vinske industrije u niskotemperaturnim eutektičkim otapalima uz primjenu softvera COSMOtherm
  Download
PDF 1.02 MB
master's thesis
Priprema polifenolnih ekstrakata iz otpada vinske industrije u niskotemperaturnim eutektičkim otapalima uz primjenu softvera COSMOtherm
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:751196
Martić, Nikolina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Martić, N. (2021). Priprema polifenolnih ekstrakata iz otpada vinske industrije u niskotemperaturnim eutektičkim otapalima uz primjenu softvera COSMOtherm (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:751196

Martić, Nikolina. "Priprema polifenolnih ekstrakata iz otpada vinske industrije u niskotemperaturnim eutektičkim otapalima uz primjenu softvera COSMOtherm." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:751196

Martić, Nikolina. "Priprema polifenolnih ekstrakata iz otpada vinske industrije u niskotemperaturnim eutektičkim otapalima uz primjenu softvera COSMOtherm." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:751196

Martić, N. (2021). 'Priprema polifenolnih ekstrakata iz otpada vinske industrije u niskotemperaturnim eutektičkim otapalima uz primjenu softvera COSMOtherm', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 29 May 2024, https://urn.nsk.hr/urn:nbn:hr:159:751196

Martić N. Priprema polifenolnih ekstrakata iz otpada vinske industrije u niskotemperaturnim eutektičkim otapalima uz primjenu softvera COSMOtherm [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2024 May 29] Available at: https://urn.nsk.hr/urn:nbn:hr:159:751196

N. Martić, "Priprema polifenolnih ekstrakata iz otpada vinske industrije u niskotemperaturnim eutektičkim otapalima uz primjenu softvera COSMOtherm", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:751196

Metadata
TitlePriprema polifenolnih ekstrakata iz otpada vinske industrije u niskotemperaturnim eutektičkim otapalima uz primjenu softvera COSMOtherm
Title (english)Polyphenolic extracts preparation from wine industry waste in natural deep eutectic solvents using COSMOtherm software
AuthorNikolina Martić
MentorIvana Radojčić Redovniković (mentor)
Committee memberKarin Kovačević-Ganić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2021-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Ekstrakti spremni za direktnu primjenu koji sadrže sekundarne biljne metabolite idealni su za primjenu u brojnim industrijama s obzirom na sve veće tendencije znanstvenika prema zelenoj ekstrakciji. Otapala pogodna za njihovu pripremu su prirodna niskotemperaturna eutektička otapala (NADES), a kao polazni materijal moguće je iskoristiti otpad vinske industrije poput komine grožđa, bogate polifenolima. U ovom radu pripravljeni su ekstrakti komine bijelog grožđa u etanolu i NADES-ima odabranima pomoću softvera COSMOtherm. Ekstraktima je određen ukupni sadržaj polifenola Folin-Ciocalteu reagensom, ORAC vrijednosti te su identificirani i kvantificirani najzastupljeniji polifenolni spojevi. Najveći prinos ekstrakcije imao je NADES betain:glukoza te je njegova bioaktivnost ispitana in vitro na Caco-2 staničnoj liniji pomoću MTS metode i DCFH-DA metode. COSMOtherm se dokazao kao pouzdan način predviđanja topljivosti spojeva u željenim otapalima te se betain:glukoza pokazao kao dostojna zamjena etanolu u postupku ekstrakcije iz biljnih materijala. Također je utvrđena prisutnost katehina, epikatehina, epigalokatehina te rutin trihidrata u komini grožđa. Optimalne koncentracije polifenola za antioksidativni i citotoksični učinak moraju još biti dalje istraživane i utvrđene kako bi polifenolni ektrakti bili učinkoviti i sigurni za ljudsku primjenu.
Abstract (english)
Ready-to-use extracts that contain secondary plant metabolites are ideal for use in many industries given the scientists' growing tendencies towards green extractions. Solvents suitable for their preparation are natural deep eutectic solvents (NADES), and as a starting material it is possible to use wine industry waste, such as grape pomace, rich in polyphenols. In this study, extracts of white grape pomace were prepared in ethanol and NADES selected by COSMOtherm. Extracts total polyphenols content was determined with Folin-Ciocalteu reagent, ORAC values were also determined and prevalent polyphenolic compounds were identified and quantified. Betaine: glucose provided the best extraction efficiency and its biological activity was evaluated in vitro on the Caco-2 cell line using the MTS and the DCFH-DA assay. COSMOtherm has proven to be reliable in predicting the solubility of compounds in given solvents, and betaine: glucose extract has proven to be a good alternative to ethanol in the extraction process. The presence of catechin, epicatechin, epigallocatechin and rutin trihydrate in grape pomace was also shown. Optimal concentrations of polyphenols in extracts for antioxidant and cytotoxic effects need to be further evaluated to be effective and safe for human consumption.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:751196
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2021-12-01 10:50:21