prikaz prve stranice dokumenta Izdvajanje mikroalge Picochlorum sp. bioflokulacijom pomoću plijesni Mortierella isabellina
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master's thesis
Izdvajanje mikroalge Picochlorum sp. bioflokulacijom pomoću plijesni Mortierella isabellina
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:283514
Strinavić, Marina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Strinavić, M. (2021). Izdvajanje mikroalge Picochlorum sp. bioflokulacijom pomoću plijesni Mortierella isabellina (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:283514

Strinavić, Marina. "Izdvajanje mikroalge Picochlorum sp. bioflokulacijom pomoću plijesni Mortierella isabellina." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:283514

Strinavić, Marina. "Izdvajanje mikroalge Picochlorum sp. bioflokulacijom pomoću plijesni Mortierella isabellina." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:283514

Strinavić, M. (2021). 'Izdvajanje mikroalge Picochlorum sp. bioflokulacijom pomoću plijesni Mortierella isabellina', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:283514

Strinavić M. Izdvajanje mikroalge Picochlorum sp. bioflokulacijom pomoću plijesni Mortierella isabellina [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:159:283514

M. Strinavić, "Izdvajanje mikroalge Picochlorum sp. bioflokulacijom pomoću plijesni Mortierella isabellina", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:283514

Metadata
TitleIzdvajanje mikroalge Picochlorum sp. bioflokulacijom pomoću plijesni Mortierella isabellina
Title (english)Harvesting of microalgae Picochlorum sp. by bioflocculation with fungus Mortierella isabellina
AuthorMarina Strinavić
MentorMirela Ivančić - Šantek (mentor)
Committee memberBlaženka Kos (predsjednik povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2021-11-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Interdisciplinary Technical Sciences
Abstract
Bioflokulacija predstavlja inovativnu, ekonomičnu i ekološki prihvatljivu metodu izdvajanja biomase mikroalge. U ovome radu provedeni su eksperimenati sa ciljem optimizacije uvjeta bioflokulacije mikroalge Picochlorum sp. s peletima plijesni Mortierella isabellina. Optimizirana je koncentracija spora plijesni te utjecaj mase plijesni, pH vrijednosti, temperature i omjera masa plijesni i mikroalge. Maksimalna uspješnost bioflokulacije iznosila je 88,47 %, postignuta pri uvjetima: 1,5 g suhe tvari plijesni u omjeru mikroalga:plijesan 1:50, pri pH vrijednosti 2 i temperaturi 30 °C. Također proveden je fototrofni šaržni uzgoj mikroalge uz sukcesivno dodavanje nutrijenata u barbotirajućoj koloni na f/2 podlozi. Uzgoj je proveden kroz fazu uzgoja i fazu nakupljanja lipida uz dodatak natrijevog bikarbonata. Posljednjeg 26. dana uzgoja postignuta koncentracija biomase iznosila je 1,68 g/L. Tijekom uzgoja praćene su promjene udjela proteina, ugljikohidrata i lipida, sastav pigmenata te profil masnih kiselina. Najzastupljenije makromolekule u biomasi bili su proteini (76,09 %), a zatim ugljikohidrati (37,69 %) i lipidi (14,18 %).
Abstract (english)
Bioflocculation is an innovative, economically and environmentally friendly method of extracting microalgae biomass. In this paper, experiments were performed with the aim of optimizing the bioflocculation conditions for the Picochlorum sp. with Mortierella isabellina pellets. Concentration of mold spores, influence of mold mass, pH value, temperature and mass ratio of mold and microalgae were optimized. The maximum efficiency of bioflocculation was 88.47 %, achieved under the following conditions: 1.5 g of mold dry matter in the ratio of microalgae: mold 1:50, at pH 2 and temperature 30 ° C. Phototrophic batch culture of microalgae was also performed with successive addition of nutrients in a bubbling column on f/2 medium. Cultivation was carried out through the culture phase and the lipid accumulation phase with the addition of sodium bicarbonate. On the last day of cultivation (26.), the achieved biomass concentration was 1.68 g/L. During cultivation, changes in the content of proteins, carbohydrates and lipids, the composition of pigments and the profile of fatty acids were monitored. The most represented macromolecules in biomass were proteins (76.09 %), followed by carbohydrates (37.69 %) and lipids (14.18 %).
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:283514
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2021-12-07 09:49:31