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master's thesis
Utjecaj omjera izvora ugljika i dušika na sastav biomase mikroalge Picochlorum sp.
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:266731
Jaković, Lorena
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Jaković, L. (2021). Utjecaj omjera izvora ugljika i dušika na sastav biomase mikroalge Picochlorum sp. (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:266731

Jaković, Lorena. "Utjecaj omjera izvora ugljika i dušika na sastav biomase mikroalge Picochlorum sp.." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:266731

Jaković, Lorena. "Utjecaj omjera izvora ugljika i dušika na sastav biomase mikroalge Picochlorum sp.." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:266731

Jaković, L. (2021). 'Utjecaj omjera izvora ugljika i dušika na sastav biomase mikroalge Picochlorum sp.', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 11 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:266731

Jaković L. Utjecaj omjera izvora ugljika i dušika na sastav biomase mikroalge Picochlorum sp. [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2024 July 11] Available at: https://urn.nsk.hr/urn:nbn:hr:159:266731

L. Jaković, "Utjecaj omjera izvora ugljika i dušika na sastav biomase mikroalge Picochlorum sp.", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:266731

Metadata
TitleUtjecaj omjera izvora ugljika i dušika na sastav biomase mikroalge Picochlorum sp.
Title (english)Effect of carbon to nitrogen ratio on biomass composition of microalga Picochlorum sp.
AuthorLorena Jaković
MentorMirela Ivančić Šantek (mentor)
Committee memberJasna Novak (predsjednik povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2021-12-03, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Fototrofnim uzgojem mikroalge Picochlorum sp. pri različitim C:N omjerima, istražen je utjecaj na njezin rast i sastav biomase. Najveća postignuta koncentracija stanica od 3,61∙107 st mLˉ¹ zabilježena je u f/2 podlozi i podlozi C:N=13,52 mol molˉ¹. Najveći udjeli proteina (75,26 %) i lipida (9,27 %) zabilježeni su u podlozi s omjerom C:N=67,64 mol molˉ¹. Najveći udio u lipidima čine zasićene masne kiseline (oko 50 %), a najmanji jednostruko nezasićene masne kiseline (oko 6-12 %). S ciljem povećane akumulacije lipida proveden je šaržni uzgoj navedene mikroalge u dvije faze- s i bez izvora dušika. Prilikom limitacije dušikom u drugoj fazi u podlogama s 1 g Lˉ¹ i 2 g Lˉ¹ NaHCO₃, ustanovljeno je smanjenje udjela proteina s 49 % na 47 % te 42 % na 31 % i ukupnog klorofila s 2,48 % na 0,67 % te 2,09 % na 0,58 %, ali je rezultirala povećanom akumulacijom lipida u sastavu biomase u podlozi sa 2 g Lˉ¹ NaHCO₃ sa 3,07 % na 10,22 %. Osim toga, ustanovljena je promjena profila masnih kiselina pri čemu je povećan udio višestruko nezasićenih masnih kiselina u f/2 podlozi i podlozi sa 1 g Lˉ¹ NaHCO₃ u odnosu na podloge koje su sadržavale izvor dušika.
Abstract (english)
Phototrophic cultivation of the microalga Picochlorum sp. at different C:N ratios, the influence on its growth and biomass composition was investigated. The highest achieved cell concentration of 3,61∙107 cells mLˉ¹ was recorded in f/2 medium and medium C:N=13,52 mol molˉ¹. The highest share of proteins (75,26 %) and lipids (9,27 %) were recorded in the medium with the ratio C:N=67,64 mol molˉ¹. The largest share in lipids are saturated fatty acids (about 50 %), and the smallest monounsaturated fatty acids (about 6-12 %). With the aim of increased lipid accumulation, batch cultivation of this microalga was carried out in two phases- with and without nitrogen sources. Nitrogen limitation in the second phase in media with 1 g Lˉ¹ and 2 g Lˉ¹ NaHCO₃, led to a decrease in protein content from 49 % to 47 % and 42 % to 31 % and total chlorophyll from 2,48 % to 0,67 % and 2,09 % to 0,58 % but resulted in increased lipid accumulation in the biomass composition in the medium with 2 g Lˉ¹ NaHCO₃ from 3,07 % to 10,22 %. In addition, a change in the obtained fatty acid profile is visible, with an increased share of polyunsaturated fatty acids in the f/2 medium and the medium with 1 g Lˉ¹ NaHCO₃ compared to the media containing the nitrogen source.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:266731
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2021-12-07 13:57:59