prikaz prve stranice dokumenta Immobilization of a whole cell catalyst for cellodextrin production
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master's thesis
Immobilization of a whole cell catalyst for cellodextrin production
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:343845
Čukelj, Iva
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Čukelj, I. (2022). Immobilization of a whole cell catalyst for cellodextrin production (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:343845

Čukelj, Iva. "Immobilization of a whole cell catalyst for cellodextrin production." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:343845

Čukelj, Iva. "Immobilization of a whole cell catalyst for cellodextrin production." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:343845

Čukelj, I. (2022). 'Immobilization of a whole cell catalyst for cellodextrin production', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 30 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:343845

Čukelj I. Immobilization of a whole cell catalyst for cellodextrin production [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 December 30] Available at: https://urn.nsk.hr/urn:nbn:hr:159:343845

I. Čukelj, "Immobilization of a whole cell catalyst for cellodextrin production", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:343845

Metadata
TitleImmobilization of a whole cell catalyst for cellodextrin production
Title (croatian)Proizvodnja celodextrina pomoću imobiliziranih stanica
AuthorIva Čukelj
MentorAnita Slavica (mentor)
Committee memberRenata Teparić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2022-02-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Enzymes sucrose phosphorylase from Bifidobacterium adolescentis (BaSP), cellobiose phosphorylase from Cellulomonas uda (CuCBP) and cellodextrin phosphorylase from Clostridium cellulosi (CcCDP) were co-expressed in E. coli BL21 (DE3) -agp⁻ pPOLY_new cells and immobilized in polyacrylamide matrix (PAM) with the aim of repeated use of immobilized cells, i.e. three enzymes in the production of soluble cellodextrins (COS). Three repetitive batch experiments were performed at three different temperatures (35, 40 and 45 °C), differing in number of cycles (9, 6 and 5, respectively), duration of each cycle (8, 12 and 6 h), cell loading (63, 40, 40 mg CDW/g PAM-particles) and cell catalyst concentration (4, 3, 4 g CDW/L). The impact of these variables has been investigated and a new experimental setup for the industrial production of COS has been proposed; six repetitive batch cycles should be performed at 40 °C, with a duration of each cycle 8-10 h, with cell loading higher than 40 mg CDW/g PAM-particles, and concentration of the cell catalyst (higher than 4 g CDW/L).
Abstract (croatian)
Enzimi saharoza fosforilaza iz Bifidobacterium adolescentis (BaSP), celobioza fosforilaza iz Cellulomonas uda (CuCBP) i celodekstrin fosforilaza iz Clostridium cellulosi (CcCDP) su ko-eksprimirani u stanicama E. coli BL21 (DE3) -agp- pPOLY_new. Stanice su zatim imobilizirane u poliakrilamidnom matriksu (PAM) s ciljem ponavljajućeg korištenja imobiliziranih stanica, odnosno ova tri enzima u proizvodnji topljivih celodekstrina (COS). Proizvodnja COS pomoću PAM imobiliziranih stanica tijekom ponavljajućih šaržnih eksperimenata provedena je na tri različite temperature (35, 40 i 45 °C) s različitim brojem ciklusa (9, 6 i 5), vremenom trajanja ciklusa (8, 12 i 6 h), udjelom stanica (63, 40 , 40 mg s.t./g immobilizata) i koncentracijom stanica (4, 3 , 4 g s.t./L). Utjecaj ovih parametara je ispitan i predloženi su sljedeći uvjeti za provođenje ponavljajućih šarži za industrijsku proizvodnju COS-a; šest ponavljajućih šaržnih ciklusa treba provesti na 40 °C, s trajanjem ciklusa 8-10 h, s većim udjelom stanica u poliakrilamidnom matriksu (≥ 4 g s.t./L), i koncentracijom stanica (≥ 63 s.t./g immobilizata).
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:159:343845
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2022-05-27 09:20:08