prikaz prve stranice dokumenta Mikrobna redukcija acetofenona u niskotemperaturnim eutektičkim otapalima
Embargo period 2025-02-03
master's thesis
Mikrobna redukcija acetofenona u niskotemperaturnim eutektičkim otapalima
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:676009
Demir, Karla
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Demir, K. (2022). Mikrobna redukcija acetofenona u niskotemperaturnim eutektičkim otapalima (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:676009

Demir, Karla. "Mikrobna redukcija acetofenona u niskotemperaturnim eutektičkim otapalima." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:676009

Demir, Karla. "Mikrobna redukcija acetofenona u niskotemperaturnim eutektičkim otapalima." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:676009

Demir, K. (2022). 'Mikrobna redukcija acetofenona u niskotemperaturnim eutektičkim otapalima', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 23 May 2024, https://urn.nsk.hr/urn:nbn:hr:159:676009

Demir K. Mikrobna redukcija acetofenona u niskotemperaturnim eutektičkim otapalima [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 May 23] Available at: https://urn.nsk.hr/urn:nbn:hr:159:676009

K. Demir, "Mikrobna redukcija acetofenona u niskotemperaturnim eutektičkim otapalima", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:676009

Metadata
TitleMikrobna redukcija acetofenona u niskotemperaturnim eutektičkim otapalima
Title (english)Microbial reduction of acetophenone in deep eutectic solvents
AuthorKarla Demir
MentorMarina Cvjetko Bubalo (mentor)
Committee memberIvana Radojčić Redovniković (predsjednik povjerenstva)
Committee memberJasna Novak (član povjerenstva)
Committee memberAndreja Leboš-Pavunc (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2022-09-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
S obzirom na ubrzani porast industrijalizacije i potrebu za stvaranjem održivijih procesa, razvijen je koncept zelene kemije koji se oslanja na primjenu obnovljivih sirovina, alternativnih otapala i biokatalizatora za pretvorbu organskih spojeva u biotransformacijskim procesima. Stoga, u upotrebu sve više ulazi primjena eutektičkih otapala kao pogodnih reakcijskih medija za dobivanje enantiomerno čistih spojeva. U ovom radu pripremljena su nova niskotemperaturna eutektička otapala na bazi osmolita te su fizikalno-kemijskom karakterizacijom određena njihova karakteristična svojstva. Isto tako, provedena je enantioselektivna redukcija različito supstituiranih acetofenona u optički aktivne alkohole upotrebom bakterija mliječne kiseline Limosilactobacillus fermentum MC1 i Levilactobacillus brevis MB1, nakon čega je provedena optimizacija redukcije acetofenona kao modelnog supstrata i L. brevis MB1 kao pogodnog biokatalizatora u niskotemperaturnom eutektičkom otapalu. Biokatalitička redukcija acetofenona u otapalu betain : malična kiselina : glukoza s 30 %-tnim masenim udjelom vode rezultirala je većim enantiomernim viškom u odnosu na istu provedenu u hranjivoj podlozi.
Abstract (english)
Considering the accelerated growth of industrialization and the need to create more sustainable processes, the concept of green chemistry was developed. It relies on the use of renewable raw materials, alternative solvents and biocatalysts for the conversion of organic compounds in biotransformation processes. Therefore, the application of eutectic solvents as suitable reaction media for obtaining enantiomerically pure compounds is increasingly being used. In this work, new osmolyte-based deep eutectic solvents were prepared and their characteristic properties were determined by physico-chemical characterization. Likewise, the enantioselective reduction of differently substituted acetophenones into optically active alcohols was carried out using lactic acid bacteria Limosilactobacillus fermentum MC1 and Levilactobacillus brevis MB1, after which the optimization of the reduction of acetophenone as a model substrate and L. brevis MB1 as a suitable biocatalyst in a deep eutectic solvent was carried out. The biocatalytic reduction of acetophenone in the betaine : malic acid : glucose solvent with a 30% water content (w/w) resulted in a higher enantiomeric excess compared to the same one carried out in the nutrient medium.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:676009
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-02-03
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2023-01-19 14:58:09