prikaz prve stranice dokumenta Određivanje biološkog potencijala frakcija morske makroalge Cystoseira compressa baziranog na pigmentima i njihovim derivatima
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master's thesis
Određivanje biološkog potencijala frakcija morske makroalge Cystoseira compressa baziranog na pigmentima i njihovim derivatima
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:232645
Begić, Karolina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Food Engineering
Laboratory for Technology of Fruits and Vegetables Preservation and Processing

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Begić, K. (2022). Određivanje biološkog potencijala frakcija morske makroalge Cystoseira compressa baziranog na pigmentima i njihovim derivatima (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:232645

Begić, Karolina. "Određivanje biološkog potencijala frakcija morske makroalge Cystoseira compressa baziranog na pigmentima i njihovim derivatima." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:232645

Begić, Karolina. "Određivanje biološkog potencijala frakcija morske makroalge Cystoseira compressa baziranog na pigmentima i njihovim derivatima." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:232645

Begić, K. (2022). 'Određivanje biološkog potencijala frakcija morske makroalge Cystoseira compressa baziranog na pigmentima i njihovim derivatima', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 03 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:232645

Begić K. Određivanje biološkog potencijala frakcija morske makroalge Cystoseira compressa baziranog na pigmentima i njihovim derivatima [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 July 03] Available at: https://urn.nsk.hr/urn:nbn:hr:159:232645

K. Begić, "Određivanje biološkog potencijala frakcija morske makroalge Cystoseira compressa baziranog na pigmentima i njihovim derivatima", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:232645

Metadata
TitleOdređivanje biološkog potencijala frakcija morske makroalge Cystoseira compressa baziranog na pigmentima i njihovim derivatima
Title (english)Determination of biological potential of Cystoseira compressa macroalgal fractions based on pigments and their derivatives
AuthorKarolina Begić
MentorIvona Elez Garofulić (mentor)
Committee memberVerica Dragović-Uzelac (predsjednik povjerenstva)
Committee memberSanja Babić (član povjerenstva)
Committee memberMaja Repajić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Food Engineering)
(Laboratory for Technology of Fruits and Vegetables Preservation and Processing)
Zagreb
Defense date and country2022-09-22, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Morske makroalge predstavljaju bogat izvor strukturno različitih i biološki aktivnih molekula. Zbog uravnotežene nutritivne vrijednosti odlični su kandidati za implementaciju u prehrambenu industriju. Unutar ovog rada ispitan je biološki potencijal dviju nepolarnih (metanolna i diklormetanska) frakcija smeđe makroalge Cystoseira compressa. Antioksidativna aktivnost utvrđena je pomoću tri in vitro metode (ORAC, FRAP i DPPH) te potvrđena in vivo pomoću živog embrija zebrice Danio rerio. Inhibicija kolagenaze utvrđena je kod obje frakcije, s nešto većim postotkom zabilježenim kod metanolne frakcije. Diklormetanska frakcija potaknula je bržu regeneraciju amputirane repne peraje kod ličinaka zebrice Danio rerio, dok kod metanolne frakcije takav učinak nije zabilježen. Testom embriotoksičnosti na embrijima zebrice utvrđene su koncentracije testiranih frakcija bez toksičnog učinka. Usporedbom biološke aktivnosti s rezultatima kemijske analize, može se zaključiti kako su fukoksantin, feoforbid a i feofitini a i b, detektirani u obje ispitivane frakcije, jedni od glavnih spojeva odgovornih za dokazane pozitivne učinke. Dobiveni rezultati pokazuju kako makroalga Cystoseira compressa predstavljaja prirodan izvor bioaktivnih molekula, posebice pigmenata i njihovih derivata, koje se mogu primijeniti u prehrambenoj i kozmetičkoj industriji.
Abstract (english)
Marine macroalgae are a rich source of structurally diverse and biologically active molecules. Due to their balanced nutritional value, they are excellent candidates for implementation in the food industry. Within this work, the biological potential of two non-polar (methanolic and dichloromethane) fractions of brown macroalga Cystoseira compressa was examined. Antioxidant activity was determined using three in vitro methods (ORAC, FRAP, and DPPH) and confirmed in vivo using zebrafish Danio rerio embryos. Collagenase inhibition was confirmed on both fractions, with a slightly higher percentage recorded on the methanolic fraction. The dichloromethane fraction stimulated rapid regeneration of the amputated tail fin in Danio rerio zebrafish larvae, while no such effect was recorded on the methanolic fraction. The embryotoxicity test on zebrafish embryos determined the concentration of tested fractions with no toxic effects. By comparing the biological activity with the results of the chemical analysis, it can be concluded that fucoxanthin, pheophorbid a and pheophytins a and b, detected in both tested fractions, are one of the main compounds responsible for the proven beneficial effects. The obtained results show that Cystoseira compressa represents a natural source of bioactive molecules, especially pigments and their derivates, that can be used in the food and cosmetic industry.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:232645
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2023-03-15 11:35:46