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master's thesis
Stabilizacija lizozima u niskotemperaturnim eutektičkim otapalima na bazi osmolita
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:665228
Lacić, Petra
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Lacić, P. (2023). Stabilizacija lizozima u niskotemperaturnim eutektičkim otapalima na bazi osmolita (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:665228

Lacić, Petra. "Stabilizacija lizozima u niskotemperaturnim eutektičkim otapalima na bazi osmolita." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:665228

Lacić, Petra. "Stabilizacija lizozima u niskotemperaturnim eutektičkim otapalima na bazi osmolita." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:665228

Lacić, P. (2023). 'Stabilizacija lizozima u niskotemperaturnim eutektičkim otapalima na bazi osmolita', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 28 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:665228

Lacić P. Stabilizacija lizozima u niskotemperaturnim eutektičkim otapalima na bazi osmolita [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 June 28] Available at: https://urn.nsk.hr/urn:nbn:hr:159:665228

P. Lacić, "Stabilizacija lizozima u niskotemperaturnim eutektičkim otapalima na bazi osmolita", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:665228

Metadata
TitleStabilizacija lizozima u niskotemperaturnim eutektičkim otapalima na bazi osmolita
Title (english)Stabilization of lysozyme in low-temperature eutectic solvents based on osmolytes
AuthorPetra Lacić
MentorMarina Cvjetko Bubalo (mentor)
Committee memberMarina Cvjetko Bubalo (predsjednik povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2023-11-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Proučavanjem živih sustava identificiran je specifičan mehanizam zaštite organizma u stresnim uvjetima koji se oslanja na male neutralne i netoksične molekule poznate pod imenom osmoliti. Osmoliti, osim što djeluju kao obrambeni mehanizam, imaju ključnu ulogu u održavanju termodinamičke stabilnosti biomakromolekula bez narušavanja njihove funkcionalnosti. S obzirom da se smatraju "kompleksnim adaptivnim sustavima" koje karakteriziraju emergentna svojstva, nedavno je otkrivena povezanost sa niskotemperaturnim eutektičkim otapalima koja učinkovito repliciraju prirodno okruženje različitih biomolekula, a ujedno imaju slična svojstva osmolita. U ovom radu su pripremljena niskotemperaturna eutektička otapala na bazi osmolita čija je struktura identificirana infracrvenom spektroskopijom sa Fourijerovom transformacijom, te je provedena karakterizacija njihovih svojstava. Također je ispitan utjecaj osmoDES-ova na termodinamičku stabilnost proteina lizozima pri ekstermnim temperaturama, te na toplinski induciranu agregaciju lizozima. S obzirom na rezultate, pripremljeni osmoDES-ovi pokazuju značajan potencijal u očuvanju stabilnosti biomakronolekula te primjeni kao medija za pohranu u usporedbi sa konvencionalnim puferima
Abstract (english)
By studying living systems, a specific mechanism for protecting the organism in stressful conditions has been identified, which relies on small neutral and non-toxic molecules known as osmolytes. Osmolytes, in addition to acting as a defensive mechanism, play a key role in maintaining the thermodynamic stability of biomacromolecules without compromising their functionality. Since they are considered as "complex adaptive systems" characterized by emergent properties, a recent connection with low-temperature eutectic solvents has been discovered, which effectively replicate the natural environment of different biomolecules while also having similar properties to osmolytes. In this study, low-temperature eutectic solvents based on osmolytes were prepared, whose structure was identified by infrared spectroscopy with Fourier transformation, and their properties characterized. The impact of osmoDESs on the thermodynamic stability of lysozyme proteins at extreme temperatures, as well as on the heat-induced aggregation of lysozymes, was also examined. Given the results, prepared osmoDESs show significant potential in preserving the stability of biomacromolecules and their application as a medium for storage compared to conventional buffers.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:665228
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-04-02 10:32:43