prikaz prve stranice dokumenta Characterisation of filamentous fungi isolated from noble rotten grape berries
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master's thesis
Characterisation of filamentous fungi isolated from noble rotten grape berries
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:827410
Dobrotić, Dominik
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Dobrotić, D. (2022). Characterisation of filamentous fungi isolated from noble rotten grape berries (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:827410

Dobrotić, Dominik. "Characterisation of filamentous fungi isolated from noble rotten grape berries." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:827410

Dobrotić, Dominik. "Characterisation of filamentous fungi isolated from noble rotten grape berries." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:827410

Dobrotić, D. (2022). 'Characterisation of filamentous fungi isolated from noble rotten grape berries', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 02 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:827410

Dobrotić D. Characterisation of filamentous fungi isolated from noble rotten grape berries [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 June 02] Available at: https://urn.nsk.hr/urn:nbn:hr:159:827410

D. Dobrotić, "Characterisation of filamentous fungi isolated from noble rotten grape berries", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:827410

Metadata
TitleCharacterisation of filamentous fungi isolated from noble rotten grape berries
Title (croatian)Karakterizacija filamentoznih gljiva izoliranih sa bobica grožđa plemenite truleži
AuthorDominik Dobrotić
MentorVladimir Mrša (mentor)
Committee memberVladimir Mrša (predsjednik povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2022-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Botrytised wines are wines that have been produced by adding noble rotten berries during fermentation. Main filamentous fungi responsible for the noble rot of grape berries is Botrytis cinerea. However, on grape berries B. cinerea is accompanied by other indigenous filamentous fungi that could affect the noble rotting process. Those fungi need to be identified to research the relationship between them and the progression of the noble rot process. Main purpose of this thesis was to identify selected isolates of filamentous fungi using morphology and molecular methods such as ARDRA and ITS sequencing. MALDI-ToF method was tried to determine if it could be used for reliable identification and RAPD method was used to determine if there is any genetic variance between the Botrytis isolates. Identified fungi were B. cinerea, fungi from the genus Alternaria, Cladosporium, Aspergillus and Penicillium. MALDI-ToF proved to be a good alternative as a method, although it is not as effective as ITS sequencing and the RAPD method indicated that there might be some genetic variability between the B. cinerea isolates.
Abstract (croatian)
Botritizirana vina su vina koja su bila proizvedena dodavanjem bobica grožđa plemenite truleži tijekom fermentacije. Glavna filamentozna gljiva odgovorna za plemenito truljenje grožđa je Botrytis cinerea. Međutim, na bobicama grožđa prisutne su i druge filamentozne gljive zajedno sa B. cinerea i one bi mogle imati utjecaj na proces plemenitog truljenja. Potrebno je identificirati te gljive kako bi se moglo provesti istraživanje utjecaja tih gljiva i progresije procesa plemenitog truljenja. Cilj ovog diplomskog rada je bila identifikacija izolata filamenoznih gljiva koristeći morfologiju i molekularne metode poput ARDRA i ITS sekvencioniranje. MALDI-ToF metoda je isprobana kako bi se odredilo da li bi se mogla koristiti za pouzdanu identifikaciju i RAPD metoda je bila korištena kako bi se odredilo da li postoji genetička razlika između izoliranih Botrytis sojeva. Identificirane gljive su bile B. cinerea, gljive roda Alternaria, Cladosporium, Aspergillus i Penicillium. MALDI-ToF se pokazala kao dobra alternativna metoda, iako nije bila efektivna kao ITS sekvencionijanje. RAPD metoda je ukazala da može biti neka genetička raznolikost između izolata B.cinerea.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:159:827410
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2024-04-29 09:51:29