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master's thesis
Kompetitivna ekskluzija patogena sojem producentom egzopolisaharida Limosilactobacillus fermentum MC1
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:785892
Knez, Petra
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Knez, P. (2023). Kompetitivna ekskluzija patogena sojem producentom egzopolisaharida Limosilactobacillus fermentum MC1 (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:785892

Knez, Petra. "Kompetitivna ekskluzija patogena sojem producentom egzopolisaharida Limosilactobacillus fermentum MC1." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:785892

Knez, Petra. "Kompetitivna ekskluzija patogena sojem producentom egzopolisaharida Limosilactobacillus fermentum MC1." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:785892

Knez, P. (2023). 'Kompetitivna ekskluzija patogena sojem producentom egzopolisaharida Limosilactobacillus fermentum MC1', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 28 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:785892

Knez P. Kompetitivna ekskluzija patogena sojem producentom egzopolisaharida Limosilactobacillus fermentum MC1 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 June 28] Available at: https://urn.nsk.hr/urn:nbn:hr:159:785892

P. Knez, "Kompetitivna ekskluzija patogena sojem producentom egzopolisaharida Limosilactobacillus fermentum MC1", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:785892

Metadata
TitleKompetitivna ekskluzija patogena sojem producentom egzopolisaharida Limosilactobacillus fermentum MC1
Title (english)Competitive exclusion of pathogens by the exopolysaccharide-producing Limosilactobacillus fermentum MC1 strain
AuthorPetra Knez
MentorAndreja Leboš Pavunc (mentor)
Committee memberJasna Novak (predsjednik povjerenstva)
Committee memberKsenija Durgo (član povjerenstva)
Committee memberMarina Cvjetko Bubalo (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2023-07-06, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj ovog rada bio je ispitati kompetitivnu ekskluziju test-mikroorganizma Escherichia coli 3014 probiotičkim sojem Limosilactobacillus fermentum MC1, producentom egzoplisaharida (EPS), korištenjem Caco-2 stanične linije u in vitro uvjetima. Također je ispitan utjecaj različitih koncentracija sintetitziranih EPS-a na kompetitivnu ekskluziju. Proučavan je i utjecaj mikroinkapsulacije soja MC1 u alginatu, uz dodatak fruktooligosaharida ili galaktooligosaharida, na preživljavanje stanica soja MC1 u procesu liofilizacije, izlaganja simuliranim uvjetima gastrointestinalnog trakta te skladištenja. Rezultati su pokazali da EPS kojeg sintetizira L. fermentum MC1 smanjuje adhezivna svojstva E. coli, s time da veća koncentracija podrazumijeva i snažnije djelovanje. Najveća antiadhezivna svojstva pokazao je uzorak koji je sadržavao 1 mg mL-1 EPS-a. Mikroinkapsulacija u natrijevom alginatu pokazala se kao dobra tehnika za zaštitu MC1 soja. Dodatak oligosaharida soju MC1 osigurava bolje preživljavanje liofilizacije te simuliranih uvjeta gastrointestinalnog trakta i tijekom skladištenja, s tim da su se galaktooligosaharidi u gotovo svim segmentima istraživanja pokazali kao bolji izbor prebiotika nego fruktooligosaharidi.
Abstract (english)
The aim of this work was to examine the competitive exclusion of the test microorganism Escherichia coli 3014 by the probiotic strain Limosilactobacillus fermentum MC1, an exopolysaccharide (EPS) producer, using the Caco-2 cell line in vitro. The influence of different concentrations of synthesized EPSs on competitive exclusion was also examined. The effect of microencapsulation of MC1 strain in alginate, with the addition of fructooligosaccharides or galactooligosaccharides, on the survival of MC1 cells during the lyophilization process, simulated gastrointestinal tract (GIT) conditions and storage was also studied. The results showed that EPSs synthesized by L. fermentum MC1 reduce the adhesive properties of E. coli, with a higher concentration implying a stronger effect. The sample containing 1 mg mL-1 EPS showed the highest anti-adhesive properties. Microencapsulation in sodium alginate proved to be a good technique for the protection of the MC1 strain. The addition of oligosaccharides to MC1strain ensures better survival of lyophilization and simulated GIT conditions and storage, with the fact that galactooligosaccharides proved to be a better choice of prebiotics than fructooligosaccharides in almost all segments of the research.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:785892
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-04-29 11:15:50