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master's thesis
Uzgoj plijesni Mucor indicus DSM 2185 pri različitim uvjetima aeracije i iskorištavanje otpadne prevrele podloge nesumpornim ljubičastim bakterijama
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:033925
Kovač, Ivan
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Kovač, I. (2023). Uzgoj plijesni Mucor indicus DSM 2185 pri različitim uvjetima aeracije i iskorištavanje otpadne prevrele podloge nesumpornim ljubičastim bakterijama (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:033925

Kovač, Ivan. "Uzgoj plijesni Mucor indicus DSM 2185 pri različitim uvjetima aeracije i iskorištavanje otpadne prevrele podloge nesumpornim ljubičastim bakterijama." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:033925

Kovač, Ivan. "Uzgoj plijesni Mucor indicus DSM 2185 pri različitim uvjetima aeracije i iskorištavanje otpadne prevrele podloge nesumpornim ljubičastim bakterijama." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:033925

Kovač, I. (2023). 'Uzgoj plijesni Mucor indicus DSM 2185 pri različitim uvjetima aeracije i iskorištavanje otpadne prevrele podloge nesumpornim ljubičastim bakterijama', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 04 August 2024, https://urn.nsk.hr/urn:nbn:hr:159:033925

Kovač I. Uzgoj plijesni Mucor indicus DSM 2185 pri različitim uvjetima aeracije i iskorištavanje otpadne prevrele podloge nesumpornim ljubičastim bakterijama [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 August 04] Available at: https://urn.nsk.hr/urn:nbn:hr:159:033925

I. Kovač, "Uzgoj plijesni Mucor indicus DSM 2185 pri različitim uvjetima aeracije i iskorištavanje otpadne prevrele podloge nesumpornim ljubičastim bakterijama", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:033925

Metadata
TitleUzgoj plijesni Mucor indicus DSM 2185 pri različitim uvjetima aeracije i iskorištavanje otpadne prevrele podloge nesumpornim ljubičastim bakterijama
Title (english)Cultivation of mold Mucor indicus DSM 2185 under different aeration conditions and utilization of waste substrate by non-sulfur purple bacteria
AuthorIvan Kovač
MentorMario Novak (mentor)
MentorNenad Marđetko (pomoć pri izradi, neposredni voditelj)
Committee memberAntonija Trontel (predsjednik povjerenstva)
Committee memberAndreja Leboš-Pavunc (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2023-07-04, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj ovog rada bio je ispitati utjecaj različitih protoka zraka pri uzgoju plijesni Mucor indicus DSM 2185 na dva hidrolizata kukuruznih oklasaka u barbotirajućem bioreaktoru te istražiti mogućnost iskorištenja otpadnih prevrelih podloga za uzgoj fototrofnih nesumpornih ljubičastih bakterija. Uzgojem plijesni u 1 L podloge pri protoku zraka od 0,5 L/min na kiselinskom hidrolizatu proizvedeno je 5,07 g/L etanola i 5 g biomase s 8,3 % masnih kiselina, a na enzimskom hidrolizatu proizvedeno je 11,7 g/L etanola i 10,8 g biomase s 13,6 % masnih kiselina. Uzgoji provedeni pri 1 i 1,5 L/min zraka proizveli su više biomase s većim udjelom masnih kiselina s manje etanola u podlozi. U anaerobnim uvjetima proizvedeno je 22,35 g/L etanola. Na iskorištenim podlogama nakon uzgoja plijesni proveo se uzgoj bakterija Rhodovulum adriaticum DSM 2178 i za Rhodobacter capsulatus JCM 21090 te se pratila sinteza bakterioklorofila, te je napravljena analiza masnih kiselina u izoliranoj biomasi.
Abstract (english)
The aim of this work was to examine the influence of different air flows during the cultivation of the mold Mucor indicus DSM 2185 on corn cob hydrolysates in a bubbling bioreactor and to investigate the use of waste media for the cultivation of phototrophic non-sulfur purple bacteria. Mold grown in 1 L of acid hydrolyzate at an air flow of 0,5 L/min produced 5,07 g/L of ethanol and 5 g of biomass with 8,3% fatty acids whereas 11,7 g/L of ethanol and 10,8 g of biomass with 13,6% fatty acids were produced on the enzymatic hydrolyzate. More biomass and fatty acids were produced with less ethanol at higher airflows. Under anaerobic conditions 22,35 g/L of ethanol was produced. Rhodovulum adriaticum DSM 2178 and Rhodobacter capsulatus JCM 21090 were cultivated on the waste media. The synthesis of bacteriochlorophyll was monitored and an analysis of fatty acids was made.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:033925
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-04-30 10:16:00