prikaz prve stranice dokumenta Biological evaluation of lanthanide-based near-infrared imaging agents for the specific recognition of HER2 protein in a breast cancer model
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master's thesis
Biological evaluation of lanthanide-based near-infrared imaging agents for the specific recognition of HER2 protein in a breast cancer model
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:426569
Ćutuk, Petra
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Ćutuk, P. (2023). Biological evaluation of lanthanide-based near-infrared imaging agents for the specific recognition of HER2 protein in a breast cancer model (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:426569

Ćutuk, Petra. "Biological evaluation of lanthanide-based near-infrared imaging agents for the specific recognition of HER2 protein in a breast cancer model." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:426569

Ćutuk, Petra. "Biological evaluation of lanthanide-based near-infrared imaging agents for the specific recognition of HER2 protein in a breast cancer model." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:426569

Ćutuk, P. (2023). 'Biological evaluation of lanthanide-based near-infrared imaging agents for the specific recognition of HER2 protein in a breast cancer model', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 15 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:426569

Ćutuk P. Biological evaluation of lanthanide-based near-infrared imaging agents for the specific recognition of HER2 protein in a breast cancer model [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 June 15] Available at: https://urn.nsk.hr/urn:nbn:hr:159:426569

P. Ćutuk, "Biological evaluation of lanthanide-based near-infrared imaging agents for the specific recognition of HER2 protein in a breast cancer model", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:426569

Metadata
TitleBiological evaluation of lanthanide-based near-infrared imaging agents for the specific recognition of HER2 protein in a breast cancer model
Title (croatian)Biološka procjena agenasa temeljenih na lantanidima u bliskom infracrvenom području za specifično prepoznavanje HER2 proteina u modelu raka dojke
AuthorPetra Ćutuk
MentorIgor Stuparević (mentor)
Committee memberVišnja Besendorfer (član povjerenstva)
Committee memberBojan Žunar (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2023-07-03, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Breast cancer (BC) is one of the most common malignancies worldwide and a leading cause of death among women. The HER2 positive breast cancer (overexpressing HER2 receptor) represents a very aggressive subtype and constitutes 15-20% of BC cases. Here we report about the creation of new diagnostic tool based on polystyrene beads incorporating Ln-based metallacrowns that were biologically targeted in a reaction of conjugation with Trastuzumab (Trz). The bioconjugation reaction was performed under various conditions. The resultinganti-HER2 conjugated beads were tested in vitro in studies of affinity, cytotoxicity and proliferation. We managed to do successful bioconjugation of Trz to polystyrene beads under optimized conditions. The conjugated compound exhibited preserved binding affinity for HER2 and showed specific interactions with HER2- overexpressing cells, the conjugate did not display extensive cytotoxic effects but inhibited the proliferation of HER2-overexpressing cells. These findings suggest the potential of Trz-beads as a targeted diagnostic tool for HER2-positive breast cancer.
Abstract (croatian)
Rak dojke (BC) jedna je od najčešćih zloćudnih bolesti u svijetu i vodeći uzrok smrti kod žena. HER2 pozitivan rak dojke (prekomjerna ekspresija HER2 receptora) predstavlja vrlo agresivan podtip i čini 15-20% slučajeva raka dojke. U ovom diplomskom radu se izvještava o razvoju dijagnostičkog alata temeljenog na polistirenskim kuglicama koje sadrže „metallacrowns“ na bazi lantanida na koje je vezan trastuzumab (Trz) u reakciji biološke konjugacije. Dobivene anti-HER2 konjugirane kuglice testirane su in vitro u studijama afiniteta, citotoksičnosti i proliferacije. Konjugirani spoj pokazao je očuvani afinitet vezanja za HER2 protein i specifične interakcije sa stanicama s prekomjernom ekspresijom HER2, bez značajnijeg citotoksičnog učinka dok inhibira proliferaciju stanica s prekomjernom ekspresijom HER2 receptora. Ovi nalazi sugeriraju potencijal Trz-kuglica kao ciljanog dijagnostičkog alata za HER2- pozitivan rak dojke.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:159:426569
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0) icon
Created on2024-05-16 08:32:58