prikaz prve stranice dokumenta Biological evaluation of lanthanide-based near-infrared imaging agents for the specific recognition of HER2 protein in a breast cancer model
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diplomski rad
Biological evaluation of lanthanide-based near-infrared imaging agents for the specific recognition of HER2 protein in a breast cancer model
Zagreb: Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet, 2023. urn:nbn:hr:159:426569
Ćutuk, Petra
Sveučilište u Zagrebu
Prehrambeno-biotehnološki fakultet
Zavod za kemiju i biokemiju
Laboratorij za biokemiju

Institucijski repozitorij: Repozitorij Prehrambeno-biotehnološkog fakulteta u Zagrebu

Citirajte ovaj rad

Ćutuk, P. (2023). Biological evaluation of lanthanide-based near-infrared imaging agents for the specific recognition of HER2 protein in a breast cancer model (Diplomski rad). Zagreb: Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet. Preuzeto s https://urn.nsk.hr/urn:nbn:hr:159:426569

Ćutuk, Petra. "Biological evaluation of lanthanide-based near-infrared imaging agents for the specific recognition of HER2 protein in a breast cancer model." Diplomski rad, Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet, 2023. https://urn.nsk.hr/urn:nbn:hr:159:426569

Ćutuk, Petra. "Biological evaluation of lanthanide-based near-infrared imaging agents for the specific recognition of HER2 protein in a breast cancer model." Diplomski rad, Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet, 2023. https://urn.nsk.hr/urn:nbn:hr:159:426569

Ćutuk, P. (2023). 'Biological evaluation of lanthanide-based near-infrared imaging agents for the specific recognition of HER2 protein in a breast cancer model', Diplomski rad, Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet, citirano: 21.06.2024., https://urn.nsk.hr/urn:nbn:hr:159:426569

Ćutuk P. Biological evaluation of lanthanide-based near-infrared imaging agents for the specific recognition of HER2 protein in a breast cancer model [Diplomski rad]. Zagreb: Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet; 2023 [pristupljeno 21.06.2024.] Dostupno na: https://urn.nsk.hr/urn:nbn:hr:159:426569

P. Ćutuk, "Biological evaluation of lanthanide-based near-infrared imaging agents for the specific recognition of HER2 protein in a breast cancer model", Diplomski rad, Sveučilište u Zagrebu, Prehrambeno-biotehnološki fakultet, Zagreb, 2023. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:159:426569

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:159:426569
Podaci o radu
NaslovBiological evaluation of lanthanide-based near-infrared imaging agents for the specific recognition of HER2 protein in a breast cancer model
Naslov (hrvatski)Biološka procjena agenasa temeljenih na lantanidima u bliskom infracrvenom području za specifično prepoznavanje HER2 proteina u modelu raka dojke
AutorPetra Ćutuk
MentorIgor Stuparević (mentor)
Član povjerenstvaVišnja Besendorfer (član povjerenstva)
Član povjerenstvaBojan Žunar (član povjerenstva)
Ustanova koja je dodijelila
akademski / stručni stupanj		Sveučilište u Zagrebu
Prehrambeno-biotehnološki fakultet
(Zavod za kemiju i biokemiju)
(Laboratorij za biokemiju)
Zagreb
Datum i država obrane2023-07-03, Hrvatska
Znanstveno / umjetničko
područje, polje i grana		BIOTEHNIČKE ZNANOSTI
Biotehnologija
Sažetak
Breast cancer (BC) is one of the most common malignancies worldwide and a leading cause of death among women. The HER2 positive breast cancer (overexpressing HER2 receptor) represents a very aggressive subtype and constitutes 15-20% of BC cases. Here we report about the creation of new diagnostic tool based on polystyrene beads incorporating Ln-based metallacrowns that were biologically targeted in a reaction of conjugation with Trastuzumab (Trz). The bioconjugation reaction was performed under various conditions. The resultinganti-HER2 conjugated beads were tested in vitro in studies of affinity, cytotoxicity and proliferation. We managed to do successful bioconjugation of Trz to polystyrene beads under optimized conditions. The conjugated compound exhibited preserved binding affinity for HER2 and showed specific interactions with HER2- overexpressing cells, the conjugate did not display extensive cytotoxic effects but inhibited the proliferation of HER2-overexpressing cells. These findings suggest the potential of Trz-beads as a targeted diagnostic tool for HER2-positive breast cancer.
Sažetak (hrvatski)
Rak dojke (BC) jedna je od najčešćih zloćudnih bolesti u svijetu i vodeći uzrok smrti kod žena. HER2 pozitivan rak dojke (prekomjerna ekspresija HER2 receptora) predstavlja vrlo agresivan podtip i čini 15-20% slučajeva raka dojke. U ovom diplomskom radu se izvještava o razvoju dijagnostičkog alata temeljenog na polistirenskim kuglicama koje sadrže „metallacrowns“ na bazi lantanida na koje je vezan trastuzumab (Trz) u reakciji biološke konjugacije. Dobivene anti-HER2 konjugirane kuglice testirane su in vitro u studijama afiniteta, citotoksičnosti i proliferacije. Konjugirani spoj pokazao je očuvani afinitet vezanja za HER2 protein i specifične interakcije sa stanicama s prekomjernom ekspresijom HER2, bez značajnijeg citotoksičnog učinka dok inhibira proliferaciju stanica s prekomjernom ekspresijom HER2 receptora. Ovi nalazi sugeriraju potencijal Trz-kuglica kao ciljanog dijagnostičkog alata za HER2- pozitivan rak dojke.
Ključne riječi
Ključne riječi (hrvatski)
Jezikengleski
URN:NBNurn:nbn:hr:159:426569
Studijski programNaziv: Molekularna biotehnologija
Vrsta studija: sveučilišni
Stupanj studija: diplomski
Akademski / stručni naziv: magistar/magistra inženjer/inženjerka molekularne biotehnologije (mag. ing. biotechn.)
Vrsta resursaTekst
Način izrade datotekeIzvorno digitalna
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Nekomercijalno-Bez prerada 3.0 (CC BY-NC-ND 3.0)
Datum i vrijeme pohrane2024-05-16 08:32:58