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master's thesis
Analiza sastava bioaktivnih peptida uslijed enzimske aktivnosti Lactococcus lactis ZGBP5-51 primjenom spektrometrije masa
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:801534
Zovko, Ljiljana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Zovko, Lj. (2023). Analiza sastava bioaktivnih peptida uslijed enzimske aktivnosti Lactococcus lactis ZGBP5-51 primjenom spektrometrije masa (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:801534

Zovko, Ljiljana. "Analiza sastava bioaktivnih peptida uslijed enzimske aktivnosti Lactococcus lactis ZGBP5-51 primjenom spektrometrije masa." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:801534

Zovko, Ljiljana. "Analiza sastava bioaktivnih peptida uslijed enzimske aktivnosti Lactococcus lactis ZGBP5-51 primjenom spektrometrije masa." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:801534

Zovko, Lj. (2023). 'Analiza sastava bioaktivnih peptida uslijed enzimske aktivnosti Lactococcus lactis ZGBP5-51 primjenom spektrometrije masa', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 10 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:801534

Zovko Lj. Analiza sastava bioaktivnih peptida uslijed enzimske aktivnosti Lactococcus lactis ZGBP5-51 primjenom spektrometrije masa [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 July 10] Available at: https://urn.nsk.hr/urn:nbn:hr:159:801534

Lj. Zovko, "Analiza sastava bioaktivnih peptida uslijed enzimske aktivnosti Lactococcus lactis ZGBP5-51 primjenom spektrometrije masa", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:801534

Metadata
TitleAnaliza sastava bioaktivnih peptida uslijed enzimske aktivnosti Lactococcus lactis ZGBP5-51 primjenom spektrometrije masa
Title (english)Analysis of the composition of bioactive peptides due to the enzymatic activity of Lactococcus lactis ZGBP5-51 using mass spectrometry
AuthorLjiljana Zovko
MentorJasna Novak (mentor)
MentorAna Butorac (komentor)
MentorKatarina Butorac (pomoć pri izradi, neposredni voditelj)
Committee memberBlaženka Kos (predsjednik povjerenstva)
Committee memberAna Butorac (član povjerenstva)
Committee memberAnita Horvatić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2023-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj ovog diplomskog rada bila je analiza bioaktivnih peptida i aromatskih spojeva, za koje se pretpostavlja da se nakupljaju uslijed enzimske i metaboličke aktivnosti probiotičkog soja Lactococcus lactis ZGBP5-51. Primijenjenom metodom spregnute tehnike plinske kromatografije i spektrometrije masa identificirani su spojevi arome. Ustanovljeno je da su karboksilne kiseline najzastupljenija kemijska skupina u analiziranim uzorcima fermentiranog mlijeka. Osim karboksilnih kiselina, nakon primjene ZGBP5-51 u monokulturi ili u kombinaciji sa starter kulturom, identificirani su raznovrsni spojevi arome koji pripadaju ketonima, aldehidima, alkanima, fenolima, alkoholima, esterima, laktonima i sulfidima. Identifikacija bioaktivnih peptida provedena je spregnutom tehnikom tekućinske kromatografije i spektrometrije masa. Identificirani peptidi, prema literaturi, iskazuju niz bioloških aktivnosti, uključujući ACE inhibitorno, antikancerogeno, imunomodulatorno, antidijabetičko, antioksidativno te cink-vezujuće djelovanje. Ciljana analiza bioaktivnih peptida provedena je spregnutom tehnikom tekućinske kromatografije, višestrukog praćenja reakcije i spektrometrije masa. Ne postoji statistički značajna razlika između količine specifičnih peptida koju sintetiziraju korišteni bakterijski sojevi. Samo u napitku fermentiranom sa sojem ZGBP5-51 detektiran je peptid sekvence ARHPHPHLSFM iz κ-kazeina, s antioksidativnim učinkom.
Abstract (english)
The aim of this thesis was the analysis of bioactive peptides and aromatic compounds, which are assumed to accumulate due to the enzymatic and metabolic activity of the probiotic strain Lactococcus lactis ZGBP5-51. Aroma compounds were identified using the coupled technique of gas chromatography and mass spectrometry. It was found that carboxylic acids are the most represented chemical group in the analyzed samples of fermented milk. Besides carboxylic acids, various aroma compounds belonging to ketones, aldehydes, alkanes, phenols, alcohols, esters, lactones, and sulfides were identified after application of ZGBP5-51 in monoculture or in combination with starter culture. The identification of bioactive peptides was performed by a combined technique of liquid chromatography and mass spectrometry. According to the literature, the identified peptides show several biological activities, including ACE inhibitory, anticancer, immunomodulatory, antidiabetic, antioxidant, and zinc-binding activity. Targeted analysis of bioactive peptides was performed using the coupled technique of liquid chromatography, multiple reaction monitoring, and mass spectrometry. There is no statistically significant difference between the number of specific peptides synthesized by the used bacterial strains. A peptide sequence ARHPHPHLSFM from κ-casein with an antioxidant effect was detected only in the sample fermented with strain ZGBP5-51.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:801534
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-05-24 09:16:27