prikaz prve stranice dokumenta Razvoj LC-MS/MS metode za određivanje glukozinolata u listu raštike (Brassica oleracea var. acephala)
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master's thesis
Razvoj LC-MS/MS metode za određivanje glukozinolata u listu raštike (Brassica oleracea var. acephala)
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:029987
Marinović, Iva
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Food Quality Control
Laboratory for Food Quality Control

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Marinović, I. (2023). Razvoj LC-MS/MS metode za određivanje glukozinolata u listu raštike (Brassica oleracea var. acephala) (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:029987

Marinović, Iva. "Razvoj LC-MS/MS metode za određivanje glukozinolata u listu raštike (Brassica oleracea var. acephala)." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:029987

Marinović, Iva. "Razvoj LC-MS/MS metode za određivanje glukozinolata u listu raštike (Brassica oleracea var. acephala)." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:029987

Marinović, I. (2023). 'Razvoj LC-MS/MS metode za određivanje glukozinolata u listu raštike (Brassica oleracea var. acephala)', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 06 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:029987

Marinović I. Razvoj LC-MS/MS metode za određivanje glukozinolata u listu raštike (Brassica oleracea var. acephala) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 July 06] Available at: https://urn.nsk.hr/urn:nbn:hr:159:029987

I. Marinović, "Razvoj LC-MS/MS metode za određivanje glukozinolata u listu raštike (Brassica oleracea var. acephala)", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:029987

Metadata
TitleRazvoj LC-MS/MS metode za određivanje glukozinolata u listu raštike (Brassica oleracea var. acephala)
Title (english)Development of LC-MS/MS method for determination of glucosinolates in kale leaf (Brassica oleracea var. acephala)
AuthorIva Marinović
MentorMarina Krpan (mentor)
MentorNikola Major (komentor)
MentorTvrtko Karlo Kovačević (pomoć pri izradi, neposredni voditelj)
Committee memberMirjana Hruškar (predsjednik povjerenstva)
Committee memberNikola Major (član povjerenstva)
Committee memberInes Panjkota-Krbavčić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Food Quality Control)
(Laboratory for Food Quality Control)
Zagreb
Defense date and country2023-07-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Abstract
Glukozinolati su sekundarni biljni metaboliti koji sadrže sumpor, a u najvećoj su količini zastupljeni u vrstama iz porodice kupusnjača. Cilj ovoga rada bio je razviti metodu tekućinske kromatografije visoke djelotvornosti u sprezi sa spektrometrijom masa (LC-MS/MS) za određivanje pojedinih glukozinolata te primijeniti istu na analizu glukozinolata u listu raštike (Brassica oleracea var. acephala). Identificirano je i kvantificirano 14 glukozinolata; osam alifatskih, tri indolna i tri aromatska glukozinolata u šest različitih primki raštike. Prosječna koncentracija ukupnih glukozinolata kretala se između 8741 mg/kg suhe tvari do 4771 mg/kg suhe tvari. Najzastupljeniji glukozinolati bili su glukoiberin, glukobrasicin i sinigrin, dok su glukozinolati s najnižim zabilježenim koncentracijama bili glukotropeolin, epiprogoitrin i glukolimnantin.
Abstract (english)
Glucosinolates are sulfur-containing plant secondary metabolites that are mostly present in Brassicaceae. The aim of this study was to develop a method of liquid chromatography with mass spectrometry (LC-MS/MS) for determination of glucosinolates and apply it to the determination of glucosinolates in kale leaf (Brassica oleracea var. acephala). A total of 14 glucosinolates in six different kale accessions were quantified in this study, including eight aliphatic glucosinolates, three indolic glucosinolates and three aromatic glucosinolates. The average concentration of total glucosinolates ranged from 8741 mg/kg dry matter to 4771 mg/kg dry matter. The most abundant glucosinolates were glucoiberin, glucobrassicin and sinigrin, while glucosinolates with the lowest detected concentrations were glucotropaeolin, epiprogoitrin and glucolimnanthin.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:029987
Study programmeTitle: Food Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-06-07 07:11:57