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master's thesis
Utjecaj natrijeva klorida na proizvodnju lipida i karotenoida u kvascu Rhodotorula sp. MG2
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:157272
Tomić, Sara
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Tomić, S. (2023). Utjecaj natrijeva klorida na proizvodnju lipida i karotenoida u kvascu Rhodotorula sp. MG2 (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:157272

Tomić, Sara. "Utjecaj natrijeva klorida na proizvodnju lipida i karotenoida u kvascu Rhodotorula sp. MG2." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:157272

Tomić, Sara. "Utjecaj natrijeva klorida na proizvodnju lipida i karotenoida u kvascu Rhodotorula sp. MG2." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:157272

Tomić, S. (2023). 'Utjecaj natrijeva klorida na proizvodnju lipida i karotenoida u kvascu Rhodotorula sp. MG2', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 26 August 2024, https://urn.nsk.hr/urn:nbn:hr:159:157272

Tomić S. Utjecaj natrijeva klorida na proizvodnju lipida i karotenoida u kvascu Rhodotorula sp. MG2 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 August 26] Available at: https://urn.nsk.hr/urn:nbn:hr:159:157272

S. Tomić, "Utjecaj natrijeva klorida na proizvodnju lipida i karotenoida u kvascu Rhodotorula sp. MG2", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:157272

Metadata
TitleUtjecaj natrijeva klorida na proizvodnju lipida i karotenoida u kvascu Rhodotorula sp. MG2
Title (english)Influence of sodium chloride on the production of lipids and carotenoids in Rhodotorula sp. MG2 yeast
AuthorSara Tomić
MentorMirela Ivančić - Šantek (mentor)
Committee memberKsenija Markov (predsjednik povjerenstva)
Committee memberJasna Novak (član povjerenstva)
Committee memberBlaženka Kos (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2023-12-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Negativni učinci na okoliš i nestašica fosilnih goriva dovode do sve veće potražnje za alternativnim izvorima goriva i energije. Oleaginozni kvasci imaju sposobnost nakupljanja lipida koji se mogu primijeniti u proizvodnji biodizela kao alternativa naftnom dizelu. Cilj ovog rada bio je istražiti učinak osmotskog stresa na stanicu kvasca Rhodotorula sp. MG2 izazvanog dodatkom natrijeva klorida u koncentraciji od 25 do 75 g/L. Učinak stresa na stanicu praćen je određivanjem rasta i sastava stanice te udjela karotenoida i lipida. Povećanjem koncentracije natrijevog klorida u hranjivoj podlozi raste udjel lipida, proteina, karotenoida te anorganskih tvari u stanici. Najveća koncentracija karotenoida u ekstraktu od 50,40 mg/L proizvedena je dodatkom najveće koncentracije natrijeva klorida od 75 g/L. Također, najveći udio lipida u stanici od 41,3 % izmjeren je kod najveće koncentracije natrijeva klorida. Metanolski esktrakt stanične biomase te egzopolisaharidi kvasca Rhodotorula sp. MG2 nisu pokazali antibakterijski i antimikotski učinak. Kvasac također ne proizvodi soforolipide.
Abstract (english)
The negative effects on the environment and the shortage of fossil fuels lead to an increasing demand for alternative sources of fuel and energy. Oleaginous yeasts produce lipids that can be used in the production of biodiesel as an alternative to petroleum diesel. The aim of this work was to investigate the effect of osmotic stress on the yeast cell Rhodotorula sp. MG2 induced by the addition of sodium chloride in concentration in the range from 25 to 75 g/L. The effect of stress on the cell was monitored by determining cell growth and composition, as well as the proportion of lipids, proteins, carotenoids and inorganic substances in the cell. The highest concentration of carotenoids in the extract of 50,40 mg/L was produced by the addition of the highest concentration of sodium chloride of 75 g/L. Also, the highest content of lipids in the cell of 41,3 % was determined at the highest concentration of sodium chloride. Methanolic extract of cellular biomass and exopolysaccharides of the yeast Rhodotorula sp. MG2 did not show antibacterial and antifungal effects. Yeast also does not produce sophorolipids.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:157272
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-06-18 09:05:42